FGFR1 Antibody (M2F12) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB120-829

Novus Biologicals, part of Bio-Techne
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NB120-829
NB120-829SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # M2F12

Format

BSA Free

Concentration

1.0 mg/ml

View all FGFR1 Antibodies »

Product Specifications

Immunogen

Recombinant human ectodomain of FGFr1a expressed in E. coli beginning with pro23; antigen contained NH2-terminal gly-ser-pro-gly-ile and COOH-terminal glu-phe sequences.

Epitope

NH2-terminus of unique NH2-terminal Ig loop of FGFr1. Epitope is within the sequence between glu30 and ala74 of FGFr1a.

Specificity

Reacts with alpha isoform of FRFR1. Reacts with the NH2-terminus of unique NH2-terminal Ig loop of FGFr1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for FGFR1 Antibody (M2F12) - BSA Free

Western Blot: FGFR1 Antibody (M2F12)BSA Free [NB120-829]

Western Blot: FGFR1 Antibody (M2F12)BSA Free [NB120-829]

Western Blot: FGF R1 Antibody (M2F12) [NB120-829] - Total protein from Ntera-2 was separated on a 7.5% gel by SDS-PAGE. Protein was transferred to PVDF membrane, blocked and probed with 1 ug/ml of anti-FGFR1. FGFR1 protein was detected at approximately 150 kDa using an anti-mouse HRP secondary antibody.
Immunocytochemistry/ Immunofluorescence: FGFR1 Antibody (M2F12) - BSA Free [NB120-829]

Immunocytochemistry/ Immunofluorescence: FGFR1 Antibody (M2F12) - BSA Free [NB120-829]

Immunocytochemistry/Immunofluorescence: FGF R1 Antibody (M2F12) [NB120-829] - NTERA2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with FGR1 (M2F12) (NB120-829) at a 1:100 dilution overnight at 4 degrees Celsius and detected with Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.
Flow Cytometry: FGFR1 Antibody (M2F12) - BSA Free [NB120-829]

Flow Cytometry: FGFR1 Antibody (M2F12) - BSA Free [NB120-829]

Flow Cytometry: FGF R1 Antibody (M2F12) [NB120-829] - Analysis of FGF R1 in MCF-7 cells (1 x 10^6 cells/ml) stained with FGF r1 antibody (NB120-829, red peaks) at a 1:1000 dilution. Detected with a FITC conjugated goat anti-mouse IgG. Shown with mIgG2a isotype control (green). Two distinct samples shown.
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Applications for FGFR1 Antibody (M2F12) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:500 - 1:1000

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry

reported in scientific literature (PMID 8168112)

Immunohistochemistry-Frozen

1:10 - 1:500

Immunoprecipitation

1:10 - 1:500

Western Blot

1 ug/mL
Application Notes
Western blotting: 1 ug/mL recognizes 10 ng recombinant bacterial, baculo-viral, or native FGFr1. Immunoprecipitation: Native, nuclear, recombinant, and ligand-labeled FGFr1. Customers have reported difficulties using this antibody in immunocytochemistry staining (see review). This antibody does not appear to work in IHC paraffin embedded sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FGFR1

FGF activity is mediated by a family of type I transmembrane tyrosine kinases, which undergo dimerization and autophosphorylation after ligand binding. Five distinct genes encode closely related FGF receptors, FGF R1 through 5. FGF Rs contain three Ig-like domains and a stretch of acidic residues between the first and second Ig-like domains. FGF R1, 2, 3, and -4 have a cytoplasmic split tyrosine-kinase domain, but FGF R5 does not. Multiple forms of FGF R1, 2, and 3 are generated by alternative splicing

Long Name

Fibroblast Growth Factor Receptor 1

Alternate Names

CD331, FGF R1, Flt-2

Entrez Gene IDs

2260 (Human); 14182 (Mouse); 79114 (Rat)

Gene Symbol

FGFR1

Additional FGFR1 Products

Product Documents for FGFR1 Antibody (M2F12) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for FGFR1 Antibody (M2F12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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