FGFR1 [p Tyr653, p Tyr654] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13251

Novus Biologicals, part of Bio-Techne
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NBP3-13251
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Chicken (100%), Fish (100%), Rhesus Macaque (100%), Xenopus (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all FGFR1 Antibodies »

Product Summary for FGFR1 [p Tyr653, p Tyr654] Antibody

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Tyr653/Tyr654 of human FGFR1. The exact sequence is proprietary.

Modification

p Tyr653, p Tyr654

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for FGFR1 [p Tyr653, p Tyr654] Antibody

Western Blot: FGFR1 [p Tyr653, p Tyr654] Antibody [NBP3-13251]

Western Blot: FGFR1 [p Tyr653, p Tyr654] Antibody [NBP3-13251]

Western Blot: FGFR1 [p Tyr653, p Tyr654] Antibody [NBP3-13251] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FGFR1 (phospho Tyr653/654) antibody (NBP3-13251) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: FGFR1 [p Tyr653, p Tyr654] Antibody [NBP3-13251]

Western Blot: FGFR1 [p Tyr653, p Tyr654] Antibody [NBP3-13251]

Western Blot: FGFR1 [p Tyr653, p Tyr654] Antibody [NBP3-13251] - Untreated (-) and treated (+) U87-MG whole cell extracts (40 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FGFR1 (phospho Tyr653/654) antibody (NBP3-13251) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: FGFR1 [p Tyr653, p Tyr654] Antibody [NBP3-13251]

Western Blot: FGFR1 [p Tyr653, p Tyr654] Antibody [NBP3-13251]

Western Blot: FGFR1 [p Tyr653, p Tyr654] Antibody [NBP3-13251] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FGFR1 (phospho Tyr653/654) antibody (NBP3-13251) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Applications for FGFR1 [p Tyr653, p Tyr654] Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Assay dependent

Immunohistochemistry-Frozen

Assay dependent

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FGFR1

FGF activity is mediated by a family of type I transmembrane tyrosine kinases, which undergo dimerization and autophosphorylation after ligand binding. Five distinct genes encode closely related FGF receptors, FGF R1 through 5. FGF Rs contain three Ig-like domains and a stretch of acidic residues between the first and second Ig-like domains. FGF R1, 2, 3, and -4 have a cytoplasmic split tyrosine-kinase domain, but FGF R5 does not. Multiple forms of FGF R1, 2, and 3 are generated by alternative splicing

Long Name

Fibroblast Growth Factor Receptor 1

Alternate Names

CD331, FGF R1, Flt-2

Gene Symbol

FGFR1

Additional FGFR1 Products

Product Documents for FGFR1 [p Tyr653, p Tyr654] Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FGFR1 [p Tyr653, p Tyr654] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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