FGFR2 Antibody (1G3)
Novus Biologicals, part of Bio-Techne | Catalog # H00002263-M01
Key Product Details
Species Reactivity
Validated:
Human, Bovine
Cited:
Human
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 1G3
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
FGFR2 (AAH39243, 621 a.a. ~ 723 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GHRMDKPANCTNELYMMMRDCWHAVPSQRPTFKQLVEDLDRILTLTTNEEYLDLSQPLEQYSPSYPDTRSSCSSGDDSVFSPDPMPYEPCLPQYPHINGSVKT
Specificity
FGFR2 - fibroblast growth factor receptor 2 (bacteria-expressed kinase, keratinocyte growth factor receptor, craniofacial dysostosis 1, Crouzon syndrome, Pfeiffer syndrome, Jackson-Weiss syndrome). This antibody cross-reacts with human FGFR1 and human FGFR3.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for FGFR2 Antibody (1G3)
Western Blot: FGFR2 Antibody (1G3) [H00002263-M01]
Western Blot: FGFR2 Antibody (1G3) [H00002263-M01] - Analysis of FGFR2 expression in transfected 293T cell line by FGFR2 monoclonal antibody (M01), clone 1G3.Lane 1: FGFR2 transfected lysate (Predicted MW: 92 KDa).Lane 2: Non-transfected lysate.Immunohistochemistry-Paraffin: FGFR2 Antibody (1G3) [H00002263-M01]
Immunohistochemistry-Paraffin: FGFR2 Antibody (1G3) [H00002263-M01] - Analysis of monoclonal antibody to FGFR2 on formalin-fixed paraffin-embedded human stomach carcinoma tissue. Antibody concentration 5 ug/ml.
Sandwich ELISA: FGFR2 Antibody (1G3) [H00002263-M01] - Detection limit for recombinant GST tagged FGFR2 is approximately 0.03ng/ml as a capture antibody.
Applications for FGFR2 Antibody (1G3)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA. Use in Immunohistochemistry reported in scientific literature (PMID: 25834803).
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: FGFR2
Long Name
Fibroblast Growth Factor Receptor 2
Alternate Names
CD332, FGF R2
Entrez Gene IDs
2263 (Human)
Gene Symbol
FGFR2
OMIM
101200 (Human)
UniProt
Additional FGFR2 Products
Product Documents for FGFR2 Antibody (1G3)
Product Specific Notices for FGFR2 Antibody (1G3)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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