FGFR3 Antibody (JM110-33)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66840

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-66840
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JM110-33

Concentration

1 mg/ml

View all FGFR3 Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human FGFR3 aa 30-63 / 806. (SwissProt: P22607 Human; SwissProt: Q61851 Mouse; Unigene:23671 Rat)

Localization

Cell membrane, Endoplasmic reticulum, Cytoplasmic vesicle, Secreted.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FGFR3 Antibody (JM110-33)

Western Blot: FGFR3 Antibody (JM110-33) [NBP2-66840]

Western Blot: FGFR3 Antibody (JM110-33) [NBP2-66840]

Western Blot: FGFR3 Antibody (JM110-33) [NBP2-66840] - Analysis of FGFR3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: Hela cell lysateLane 2: HepG2 cell lysateLane 3: 293 cell lysateLane 4: MCF-7 cell lysate
Immunocytochemistry/ Immunofluorescence: FGFR3 Antibody (JM110-33) [NBP2-66840]

Immunocytochemistry/ Immunofluorescence: FGFR3 Antibody (JM110-33) [NBP2-66840]

Immunocytochemistry/Immunofluorescence: FGFR3 Antibody (JM110-33) [NBP2-66840] - Staining FGFR3 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: FGFR3 Antibody (JM110-33) [NBP2-66840]

Immunohistochemistry-Paraffin: FGFR3 Antibody (JM110-33) [NBP2-66840]

Immunohistochemistry-Paraffin: FGFR3 Antibody (JM110-33) [NBP2-66840] - Analysis of paraffin-embedded mouse testis tissue using anti-FGFR3 antibody. Counter stained with hematoxylin.
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Applications for FGFR3 Antibody (JM110-33)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:1,000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FGFR3

FGF activity is mediated by a family of type I transmembrane tyrosine kinases, which undergo dimerization and autophosphorylation after ligand binding. Five distinct genes encode closely related FGF receptors, FGF R1 through 5. FGF Rs contain three Ig-like domains and a stretch of acidic residues between the first and second Ig-like domains. FGF R1, 2, 3, and -4 have a cytoplasmic split tyrosine-kinase domain, but FGF R5 does not. Multiple forms of FGF R1, 2, and 3 are generated by alternative splicing

Long Name

Fibroblast Growth Factor Receptor 3

Alternate Names

CD333, CEK, FGF R3, JTK4

Gene Symbol

FGFR3

Additional FGFR3 Products

Product Documents for FGFR3 Antibody (JM110-33)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FGFR3 Antibody (JM110-33)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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