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FGFR4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84587

Novus Biologicals, part of Bio-Techne
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NBP1-84587
NBP1-84587-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%), Rat (91%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VEALDKVLLAVSEEYLDLRLTFGPYSPSGGDASSTCSSSDSVFSHDPLPLGSSSF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FGFR4 Antibody

Western Blot: FGFR4 Antibody [NBP1-84587]

Western Blot: FGFR4 Antibody [NBP1-84587]

Western Blot: FGF R4 Antibody [NBP1-84587] - Analysis in control (vector only transfected HEK293T lysate) and fGFR4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: FGFR4 Antibody [NBP1-84587]

Immunohistochemistry-Paraffin: FGFR4 Antibody [NBP1-84587]

Immunohistochemistry-Paraffin: FGF R4 Antibody [NBP1-84587] - Staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.

Applications for FGFR4 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FGFR4

FGF activity is mediated by a family of type I transmembrane tyrosine kinases, which undergo dimerization and autophosphorylation after ligand binding. Five distinct genes encode closely related FGF receptors, FGF R1 through 5. FGF Rs contain three Ig-like domains and a stretch of acidic residues between the first and second Ig-like domains. FGF R1, 2, 3, and -4 have a cytoplasmic split tyrosine-kinase domain, but FGF R5 does not. Multiple forms of FGF R1, 2, and 3 are generated by alternative splicing

Long Name

Fibroblast Growth Factor Receptor 4

Alternate Names

CD334, FGF R4

Gene Symbol

FGFR4

Additional FGFR4 Products

Product Documents for FGFR4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FGFR4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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