FHIT Antibody (104)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90283
Recombinant Monoclonal Antibody
![Western Blot: FHIT Antibody (104) [NBP2-90283]](https://resources.bio-techne.com/images/products/FHIT-Antibody-104-Western-Blot-NBP2-90283-img0001.jpg "Western Blot: FHIT Antibody (104) [NBP2-90283]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 104
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human FHIT (Uniprot#: P49789; Met1-Gln147).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for FHIT Antibody (104)
Western Blot: FHIT Antibody (104) [NBP2-90283]
Western Blot: FHIT Antibody (104) [NBP2-90283] - Anti-FHIT rabbit monoclonal antibody at 1:500 dilution. Lane A: HL-60 Whole Cell Lysate. Lysates/proteins at 30 µg per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Performed under reducing conditions. Predicted band size:17 kDa Observed band size:20 kDa.![Immunocytochemistry/ Immunofluorescence: FHIT Antibody (104) [NBP2-90283]](https://resources.bio-techne.com/images/products/FHIT-Antibody-104-Immunocytochemistry-Immunofluorescence-NBP2-90283-img0002.jpg "Immunocytochemistry/ Immunofluorescence: FHIT Antibody (104) [NBP2-90283]")
Immunocytochemistry/ Immunofluorescence: FHIT Antibody (104) [NBP2-90283]
Immunocytochemistry/Immunofluorescence: FHIT Antibody (104) [NBP2-90283] - Immunofluorescence staining of Human FHIT in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human FHIT monoclonal antibody (1:300) at 4C overnight. Then cells were stained with the Alexa Fluor(TM) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Applications for FHIT Antibody (104)
Application
Recommended Usage
ELISA
1:25000-1:50000
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Western Blot
1:500-1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: FHIT
Alternate Names
AP3A hydrolase, AP3AaseFRA3Bbis(5'-adenosyl)-triphosphatase, Diadenosine 5'-5'''-P1, Dinucleosidetriphosphatase, EC 3.6.1.29, fragile histidine triad gene, Fragile histidine triad protein, P3-triphosphate hydrolase, tumor suppressor protein
Gene Symbol
FHIT
Additional FHIT Products
Product Documents for FHIT Antibody (104)
Product Specific Notices for FHIT Antibody (104)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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