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FHIT Antibody (3D3)

Novus Biologicals, part of Bio-Techne | Catalog # H00002272-M04

Novus Biologicals, part of Bio-Techne
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H00002272-M04

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 3D3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

FHIT (AAH32336, 31 a.a. ~ 130 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VVPGHVLVCPLRPVERFHDLRPDEVADLFQTTQRVGTVVEKHFHGTSLTFSMQDGPEAGQTVKHVHVHVLPRKAGDFHRNDSIYEELQKHDKEDFPASWR

Specificity

FHIT - fragile histidine triad gene

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for FHIT Antibody (3D3)

Sandwich ELISA: FHIT Antibody (3D3) [H00002272-M04] - Detection limit for recombinant GST tagged FHIT is approximately 10ng/ml as a capture antibody.

Applications for FHIT Antibody (3D3)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: FHIT

This gene, a member of the histidine triad gene family, encodes a diadenosine 5',5'''-P1,P3-triphosphate hydrolase involved in purine metabolism. The gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. In fact, aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas.

Alternate Names

AP3A hydrolase, AP3AaseFRA3Bbis(5'-adenosyl)-triphosphatase, Diadenosine 5'-5'''-P1, Dinucleosidetriphosphatase, EC 3.6.1.29, fragile histidine triad gene, Fragile histidine triad protein, P3-triphosphate hydrolase, tumor suppressor protein

Entrez Gene IDs

2272 (Human)

Gene Symbol

FHIT

OMIM

601153 (Human)

Additional FHIT Products

Product Documents for FHIT Antibody (3D3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FHIT Antibody (3D3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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