FHL5 Antibody (1G12-D2)

Novus Biologicals, part of Bio-Techne | Catalog # H00009457-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1G12-D2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all FHL5 Antibodies »

Product Specifications

Immunogen

FHL5 (AAH21723, 1 a.a. ~ 284 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTTAHFYCQYCTASLLGKKYVLKDDSPYCVTCYDRVFSNYCEECKKPIESDSKDLCYKDRHWHEGCFKCTKCNHSLVEKPFAAKDERLLCTECYSNECSSKCFHCKRTIMPGSRKMEFKGNYWHETCFVCENCRQPIGTKPLISKESGNYCVPCFEKEFAHYCNFCKKVITSGGITFCDQLWHKECFLCSDCRKDLCEEQFMSRDDYPFCMDCYNHLYANKCVACSKPISGLTGAKFICFQDSQWHSECFNCGKCSVSLVGKGFLTQNKEIFCQKCGSGMDTDI

Reactivity Notes

Human. Other species not tested.

Specificity

FHL5 - four and a half LIM domains 5

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for FHL5 Antibody (1G12-D2)

Western Blot: FHL5 Antibody (1G12-D2) [H00009457-M01]

Western Blot: FHL5 Antibody (1G12-D2) [H00009457-M01]

Western Blot: FHL5 Antibody (1G12-D2) [H00009457-M01] - Analysis of FHL5 expression in transfected 293T cell line by FHL5 monoclonal antibody (M01), clone 1G12-D2.Lane 1: FHL5 transfected lysate(32.8 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: FHL5 Antibody (1G12-D2) [H00009457-M01]

Immunocytochemistry/ Immunofluorescence: FHL5 Antibody (1G12-D2) [H00009457-M01]

Immunocytochemistry/Immunofluorescence: FHL5 Antibody (1G12-D2) [H00009457-M01] - Analysis of monoclonal antibody to FHL5 on HepG2 cell. Antibody concentration 10 ug/ml.
Western Blot: FHL5 Antibody (1G12-D2) [H00009457-M01]

Western Blot: FHL5 Antibody (1G12-D2) [H00009457-M01]

Western Blot: FHL5 Antibody (1G12-D2) [H00009457-M01] - Western Blot analysis of FHL5 expression in HepG2 ( Cat # L019V1 ).

Applications for FHL5 Antibody (1G12-D2)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: FHL5

The protein encoded by this gene is coordinately expressed with activator of cAMP-responsive element modulator (CREM). It is associated with CREM and confers a powerful transcriptional activation function. CREM acts as a transcription factor essential for the differentiation of spermatids into mature spermatozoa. There are multiple polyadenylation sites found in this gene.

Alternate Names

activator of cAMP-responsive element modulator (CREM) in testis, Activator of cAMP-responsive element modulator in testis, Activator of CREM in testis, ACTKIAA0776, dJ393D12.2, FHL-5, FLJ33049, four and a half LIM domains 5,1700027G07Rik, four and a half LIM domains protein 5, LIM protein ACT, RP3-393D12.2

Entrez Gene IDs

9457 (Human)

Gene Symbol

FHL5

OMIM

605126 (Human)

UniProt

AAH21723 (Human)

Additional FHL5 Products

Product Documents for FHL5 Antibody (1G12-D2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FHL5 Antibody (1G12-D2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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