FHL5 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32660
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Predicted:
Chimpanzee (98%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human FHL5. The exact sequence is proprietary.
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 23127979).
Localization
Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for FHL5 Antibody
![Western Blot: FHL5 Antibody [NBP1-32660]](https://resources.bio-techne.com/images/products/FHL5-Antibody-Western-Blot-NBP1-32660-img0005.jpg "Western Blot: FHL5 Antibody [NBP1-32660]")
Western Blot: FHL5 Antibody [NBP1-32660]
Western Blot: FHL5 Antibody [NBP1-32660] - Sample (30 ug of whole cell lysate) A: 293T B: Raji 12 % SDS PAGE, antibody diluted at 1:1000.![Immunocytochemistry/ Immunofluorescence: FHL5 Antibody [NBP1-32660]](https://resources.bio-techne.com/images/products/FHL5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32660-img0003.jpg "Immunocytochemistry/ Immunofluorescence: FHL5 Antibody [NBP1-32660]")
Immunocytochemistry/ Immunofluorescence: FHL5 Antibody [NBP1-32660]
Immunocytochemistry/Immunofluorescence: FHL5 Antibody [NBP1-32660] - Paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.![Immunohistochemistry-Paraffin: FHL5 Antibody [NBP1-32660]](https://resources.bio-techne.com/images/products/FHL5-Antibody-Immunohistochemistry-Paraffin-NBP1-32660-img0004.jpg "Immunohistochemistry-Paraffin: FHL5 Antibody [NBP1-32660]")
Immunohistochemistry-Paraffin: FHL5 Antibody [NBP1-32660]
Immunohistochemistry-Paraffin: FHL5 Antibody [NBP1-32660] - SW480 xenograft , using FHL5 antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.Applications for FHL5 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: FHL5
Alternate Names
activator of cAMP-responsive element modulator (CREM) in testis, Activator of cAMP-responsive element modulator in testis, Activator of CREM in testis, ACTKIAA0776, dJ393D12.2, FHL-5, FLJ33049, four and a half LIM domains 5,1700027G07Rik, four and a half LIM domains protein 5, LIM protein ACT, RP3-393D12.2
Gene Symbol
FHL5
Additional FHL5 Products
Product Documents for FHL5 Antibody
Product Specific Notices for FHL5 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.