Fibrillarin Antibody (38F3)

Novus Biologicals, part of Bio-Techne | Catalog # NB300-269

Novus Biologicals, part of Bio-Techne
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NB300-269
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, C. elegans, Chicken, Drosophila, Equine, Plant, S. pombe, Yeast

Cited:

Human, Mouse, Insect - Drosophila, Nematode - Caenorhabditis elegans, Plant, S. pombe

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Whole-Mount, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

IF/IHC, Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry Whole-Mount, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 38F3

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Fibrillarin Antibodies »

Product Specifications

Immunogen

Yeast nuclear preparation (S. cerevisiae).

Reactivity Notes

C. elegans and plant reactivity reported in scientific literature (PMID: 24722283 and 27588463 respectively). Use in S. pombe reported in scientific literature (PMID:31855181).

Localization

Nucleus > nucleolus. Note: Fibrillar region of the nucleolus.

Marker

Nucleolar Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

34.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Fibrillarin Antibody (38F3)

Immunocytochemistry/ Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269]

Immunocytochemistry/ Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269]

Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - High magnification confocal image of HeLa cells stained with fibrillarin antibody, dilution 1:100 from concentrated tissue culture media (Green), and costained with chicken vimentin pAb (Red), dilution 1:1,000. Nuclear DNA is revealed with the DAPI stain (Blue). The fibrillarin antibody shows strong staining of nucleoli in the nucleus, while the vimentin antibody reveals cytoplasmic intermediate filaments.
Western Blot: Fibrillarin Antibody (38F3) [NB300-269]

Western Blot: Fibrillarin Antibody (38F3) [NB300-269]

Western Blot: Fibrillarin Antibody (38F3) [NB300-269] - Analysis of lysates of cell fractions probed with Fibrillarin antibody, dilution 1:500 (Green): [1] protein standard, [2] C6 cytosol, [3] C6 nuclear, [4] HEK293 cytosol, [5] HEK293 nuclear, [6] NIH-3T3 cytosol and [7] NIH-3T3 nuclear fractions. The band at 37kDa corresponds to the fibrillarin protein detected exclusively in the nuclear fractions.
Immunocytochemistry/ Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269]

Immunocytochemistry/ Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269]

Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - The nuclei of hth mutants have dispersed rDNA foci and ectopic nucleoli and produce more rRNA. A) Wild type nuclei stained with anti-Fibrillarin (red) and a specific probe that recognizes the rDNA genomic region (green). B) hth mutant nuclei stained as in A. The mutant nuclei exhibit an increased number of nucleoli with different sizes. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0120662), licensed under a CC-BY license.
View 9 more images

Applications for Fibrillarin Antibody (38F3)

Application
Recommended Usage

Flow Cytometry

1:20-1:400

Immunocytochemistry/ Immunofluorescence

1:500-1:1000

Immunohistochemistry

1:500-1:1000

Immunohistochemistry-Frozen

1:500-1:1000

Immunohistochemistry-Paraffin

1:500-1:1000

Western Blot

1:1000-1:5000
Application Notes
This Fibrillarin Antibody (38F3) is useful for Western blot, Immunocytochemistry/Immunofluorescence, Flow Cytometry and Immunohistochemistry on both paraffin-embedded and frozen sections. In WB a band can be seen at approximately 34.5 kDa. In ICC/IF, this antibody shows prominent specular nucleolar staining.

Use in IHC-WhMt and IHC-Fr reported in scientific literature (PMID: 24722283 and 21539824 respectively). Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 23542174).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 4 reviews rated 5 using NB300-269 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Supplied as concentrated hybridoma cell culture media.

Preservative

0.035% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Fibrillarin

Fibrillarin / Nop1p, was originally identified as a nucleolar protein of baker's yeast, Saccharomyces cerevisiae (accession P15646). The Nop1p protein is essential for yeast viability and is localized in the nucleoli. The human homologue of Nop1p is Fibrillarin (accession P22087), a component of the nucleolar small nuclear ribonucleoprotein (snRNP) particle. The human Fibrillarin gene is located on chromosome 19 (19q13.1). Fibrillarin proteins have been cloned and sequenced from several other species (mouse, accession P35550, Xenopus accession P22232, C. elegans accession Q22053, and S. pombe accession P35551). The N terminal 80 amino acids contain multiple copies based on the peptide RGG, and the remaining 240 amino acids consist of the Fibrillarin domain. A Fibrillarin homologue has also been identified in the genome of the archean Methanococcus (accession NC_000909). This protein lacks the RGG rich N-terminal extension but is clearly homologous to the other sequences throughout the entire Fibrillarin domain. The structure of this molecule has been determined and shown to consist of 2 extended beta-sheets flanked by 4 alpha-helixes. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a 34kDa protein which was subsequently found to be Fibrillarin. Fibrillarin is an excellent marker for the nucleolus.

Alternate Names

EC 2.1.1,34-kD nucleolar scleroderma antigen, EC 2.1.1.-, EC 2.1.1.37, FIB, FIB1,34 kDa nucleolar scleroderma antigen, fibrillarin, FLRNrRNA 2'-O-methyltransferase fibrillarin, RNA, U3 small nucleolar interacting protein 1, RNU3IP1

Entrez Gene IDs

2091 (Human); 14113 (Mouse); 292747 (Rat)

Gene Symbol

FBL

Additional Fibrillarin Products

Product Documents for Fibrillarin Antibody (38F3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Fibrillarin Antibody (38F3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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