Fibrillarin Antibody (4A4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05533

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4A4

Concentration

1 mg/ml

View all Fibrillarin Antibodies »

Product Summary for Fibrillarin Antibody (4A4)

Immunogen

Recombinant full length human Fibrillarin sequence expressed in and purified from E. coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

34.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Fibrillarin Antibody (4A4)

Western Blot: Fibrillarin Antibody [NBP3-05533]

Western Blot: Fibrillarin Antibody [NBP3-05533]

Western Blot: Fibrillarin Antibody [NBP3-05533] - Western blot analysis of different tissue and cell line lysates using Fibrillarin Antibody at a dilution of 1:2,000, in green: [1] protein standard (red), [2] rat whole brain, [3] mouse whole brain, [4] NIH-3T3 cells,[5] HEK293, [6] HeLa, and [7] SH-SY5Y cells. Strong band at ~35kDa corresponds to Fibrillarin protein seen in all cell line lysates. A much weaker band is seen in tissue lysates since Fibrillarin is more heavily expressed in rapidly dividing cells. The blot simultaneously was probed with rabbit pAb to UCHL1 at a dilution of 1:3,000, in red, revealing the ~25kDa UCHL1 protein in lysates of tissues containing neurons or cells with neuronal properties.
Immunocytochemistry/ Immunofluorescence: Fibrillarin Antibody [NBP3-05533]

Immunocytochemistry/ Immunofluorescence: Fibrillarin Antibody [NBP3-05533]

Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody [NBP3-05533] - Immunofluorescent analysis of (A) C6 rat glioma cells and (B) HEK293 human embryonic kidney cells stained with Fibrillarin Antibody at a dilution of 1:1,000 in red, in both cases costained with chicken pAb to vimentin at a dilution of 1:10,000 in green. The blue is DAPI staining of nuclear DNA. The Fibrillarin antibody detects Fibrillarin protein localized in nucleoli while the vimentin antibody produces strong staining of cytoplasmic intermediate filaments.

Applications for Fibrillarin Antibody (4A4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000-1:5000

Immunohistochemistry

1:1000-1:5000

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50% PBS, 50% glycerol

Preservative

0.035% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.

Background: Fibrillarin

Nop1p / Fibrillarin was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae (accession P15646). The Nop1p protein is essential for yeast viability and is localized in the nucleoli. The human homologue of Nop1p is fibrillarin (accession P22087) a component of the nucleolar small nuclear ribonucleoprotein (snRNP) particle. The human fibrillarin gene is located on chromosome 19 (19q13.1). Fibrillarin proteins have been cloned and sequenced from several other species (Mouse, accession P35550, Xenopus accession P22232, C. elegans accession Q22053, and S. pombe accession P35551). The N terminal 80 amino acids contain multiple copies based on the peptide RGG, and the remaining 240 amino acids consist of the fibrillarin domain. A fibrillarin homologue has also been identified in the genome of the archean Methanococcus (accession NC_000909). This protein lacks the RGG rich N-terminal extension but is clearly homologous to the other sequences throughout all of the fibrillarin domain. The structure of this molecule has been determined and shown to consist of 2 extended beta-sheets flanked by 4 alpha-helixes. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a 34kDa protein which was subsequently found to be fibrillarin. Fibrillarin is an excellent marker for the nucleolus.

Alternate Names

EC 2.1.1,34-kD nucleolar scleroderma antigen, EC 2.1.1.-, EC 2.1.1.37, FIB, FIB1,34 kDa nucleolar scleroderma antigen, fibrillarin, FLRNrRNA 2'-O-methyltransferase fibrillarin, RNA, U3 small nucleolar interacting protein 1, RNU3IP1

Gene Symbol

FBL

Additional Fibrillarin Products

Product Documents for Fibrillarin Antibody (4A4)

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Certificate of Analysis

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Product Specific Notices for Fibrillarin Antibody (4A4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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