Fibroblasts Antibody (TE-7)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-50082
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Cited:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # TE-7
Concentration
0.1 mg/ml
Product Specifications
Immunogen
Whole human thymic stoma cells (Haynes, B.F., et al. (1984). J. Exp. Med. 159(4):1149-1168).
Specificity
The antibody reacts with fibroblasts in tissue as well as cultured fibroblasts. On skin sections the antibody reacts with stratum basal epidermal cells. The antigen defined by antibody TE-7 appears to be a marker for mesodermally dervied (mesenchymal) human connective tissue and tumors derived from such tissue, e.g. fibrosarcomas (Haynes, B.F., et al. (1984). J. Exp. Med. 159(4):1149-1168). The antibody is useful for the assessment of thymic epithelial culture contamination with fibroblasts, and the identification of cells of mesodermal origin in the fetus.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Fibroblasts Antibody (TE-7)
![Immunocytochemistry/ Immunofluorescence: Fibroblasts Antibody (TE-7) [NBP2-50082]](https://resources.bio-techne.com/images/products/Fibroblasts-Antibody-TE-7-Immunocytochemistry-NBP2-50082-img0005.jpg "Immunocytochemistry/ Immunofluorescence: Fibroblasts Antibody (TE-7) [NBP2-50082]")
Immunocytochemistry/ Immunofluorescence: Fibroblasts Antibody (TE-7) [NBP2-50082]
Immunocytochemistry/Immunofluorescence: Fibroblasts Antibody (TE-7) [NBP2-50082] - MRC-5 human embryonic lung fibroblast cell line, fixation with PFA 4%, blocking with PBS 1X + 1% BSA + 0.1% Tween, primary antibody used at 1:50 in blocking buffer, O/N. Secondary antibody: goat anti mouse Alexa Fluor 594 for 1 hour at RT. Signal was detected by fluorescence microscopy at 40X. This image was submitted via customer review.![Immunohistochemistry-Paraffin: Fibroblasts Antibody (TE-7) [NBP2-50082]](https://resources.bio-techne.com/images/products/Fibroblasts-Antibody-TE-7-Immunohistochemistry-Paraffin-NBP2-50082-img0004.jpg "Immunohistochemistry-Paraffin: Fibroblasts Antibody (TE-7) [NBP2-50082]")
Immunohistochemistry-Paraffin: Fibroblasts Antibody (TE-7) [NBP2-50082]
Immunohistochemistry-Paraffin: Fibroblasts Antibody (TE-7) [NBP2-50082] - Paraffin-embedded gastrocnemius section from a patient with myopathic condition was performed overnight at 4 degrees C using 10 ug/mL.Applications for Fibroblasts Antibody (TE-7)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100-1:200
Reviewed Applications
Read 1 review rated 5 using NBP2-50082 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS with 1 mg/ml BSA
Preservative
0.07% Sodium Azide
Concentration
0.1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Fibroblasts
Alternate Names
fibro-blasts
Additional Fibroblasts Products
Product Documents for Fibroblasts Antibody (TE-7)
Product Specific Notices for Fibroblasts Antibody (TE-7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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