Fibronectin Antibody (568)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45018
![Immunohistochemistry-Paraffin: Fibronectin Antibody (568) [NBP2-45018]](https://resources.bio-techne.com/images/products/Fibronectin-Antibody-568-Immunohistochemistry-Paraffin-NBP2-45018-img0001.jpg "Immunohistochemistry-Paraffin: Fibronectin Antibody (568) [NBP2-45018]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Cited:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 568
Concentration
0.2 mg/ml
Product Specifications
Immunogen
High molecular weight proteins secreted by cultivated human fibroblasts (Uniprot: P02751)
Reactivity Notes
Use in Human reported in scientific literature (PMID:34474855)
Localization
Connective tissue matrix
Specificity
Epitope of this monoclonal antibody is located in the 8th type III repeat in the cell-binding region of fibronectin. Fibronectins are disulfide-linked, dimeric glycoproteins of ~440kDa. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectins are involved in cell adhesion, tissue organization, and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors. This monoclonal antibody is excellent for staining of formalin-fixed, paraffin-embedded tissues.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-48018)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Fibronectin Antibody (568)
Immunohistochemistry-Paraffin: Fibronectin Antibody (568) [NBP2-45018]
Immunohistochemistry-Paraffin: Fibronectin Antibody (568) [NBP2-45018] - Human Renal Cell Carcinoma stained with Fibronectin Monoclonal Antibody (568).Applications for Fibronectin Antibody (568)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
0.5 - 1.0 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Use in Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID:34474855)
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Fibronectin
Additional Fibronectin Products
Product Documents for Fibronectin Antibody (568)
Product Specific Notices for Fibronectin Antibody (568)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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