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Fibronectin Antibody (CL3730) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43942

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL3730

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P02751, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Fibronectin Antibody (CL3730) - Azide and BSA Free

Fibronectin Antibody (CL3730) - Azide and BSA Free Western Blot: Fibronectin Antibody [NBP3-43942]

Western Blot: Fibronectin Antibody [NBP3-43942]

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human cell line U-87 MG
Fibronectin Antibody (CL3730) - Azide and BSA Free Immunohistochemistry-Paraffin: Fibronectin Antibody [NBP3-43942]

Immunohistochemistry-Paraffin: Fibronectin Antibody [NBP3-43942]

Staining of human breast cancer shows strong immunoreactivity in tumor stroma.
Fibronectin Antibody (CL3730) - Azide and BSA Free Immunohistochemistry-Paraffin: Fibronectin Antibody [NBP3-43942]

Immunohistochemistry-Paraffin: Fibronectin Antibody [NBP3-43942]

Staining of human liver cancer shows strong positivity in tumor.

Applications for Fibronectin Antibody (CL3730) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
IHC-Paraffin HIER pH6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Fibronectin

Fibronectin (also known as FN1, or Fibronectin 1) is an extracellular matrix glycoprotein that binds to integrins and has a large role in cell adhesion, migration, differentiation, and growth. It plays a key role in wound healing by helping form blood clots and participates in embryonic development by guiding cell attachment and migration. Fibronectin consists of two monomers linked by disulfide bonds and binds extracellular components such as collagen fibrin and syndecans. Fibronectin has been implicated in carcinoma and tumor development while also limiting tumor cells response to treatment. Novus offers Fibronectin antibodies, ELISA Kits, lysates, and proteins

Alternate Names

CIG, ED-B, FINC, FN1, FNZ, GFND2, LETS, SMDCF

Gene Symbol

FN1

Additional Fibronectin Products

Product Documents for Fibronectin Antibody (CL3730) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Fibronectin Antibody (CL3730) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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