Fibronectin Antibody (SPM246)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32850
![Immunohistochemistry-Paraffin: Fibronectin Antibody (SPM246) [NBP2-32850]](https://resources.bio-techne.com/images/products/Fibronectin-Antibody-SPM246-Immunohistochemistry-Paraffin-NBP2-32850-img0001.jpg "Immunohistochemistry-Paraffin: Fibronectin Antibody (SPM246) [NBP2-32850]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Porcine
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM246
Concentration
0.2 mg/ml
Product Specifications
Immunogen
T-cell lymphoma biopsy
Localization
Connective tissue matrix
Specificity
Fibronectin is a soluble dimeric glycoprotein of 440kDa, which is present in cells, extracellular matrix, and blood. This monoclonal antibody reacts with the cellular as well as plasma form of fibronectin. Reportedly, after iv administration, this monoclonal antibody localizes to tumor vessels where it binds to the underlying basement. Epitope recognized by this antibody is not accessible in normal tissues to the circulating monoclonal antibody indicating that it can be used to specifically target tumor vessels in vivo. TV-1 is reportedly useful for delivering vasoactive agents to tumors to induce increased vascular permeability or blood flow prior to treatment with chemotherapeutic drugs or monoclonal antibodys.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34749)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Fibronectin Antibody (SPM246)
Immunohistochemistry-Paraffin: Fibronectin Antibody (SPM246) [NBP2-32850]
Immunohistochemistry-Paraffin: Fibronectin Antibody (SPM246) [NBP2-32850] - Formalin-fixed, paraffin-embedded human Pancreatic Adeno Carcinoma stained with Fibronectin Monoclonal Antibody (SPM246).Applications for Fibronectin Antibody (SPM246)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Fibronectin
Additional Fibronectin Products
Product Documents for Fibronectin Antibody (SPM246)
Product Specific Notices for Fibronectin Antibody (SPM246)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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