Fibronectin Antibody (SPM539)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32848
![Immunohistochemistry-Paraffin: Fibronectin Antibody (SPM539) [NBP2-32848]](https://resources.bio-techne.com/images/products/Fibronectin-Antibody-SPM539-Immunohistochemistry-Paraffin-NBP2-32848-img0001.jpg "Immunohistochemistry-Paraffin: Fibronectin Antibody (SPM539) [NBP2-32848]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM539
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Human fibronectin Purified from serum by affinity chromatography on gelatin-sepharose (Uniprot: P02751)
Localization
Connective tissue matrix
Specificity
Fibronectin is a dimeric glycoprotein of 440kDa, which is present in cells, extracellular matrix, and blood. It possesses at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectin is involved in cell adhesion, tissue organization, and wound healing. This monoclonal antibody is directed against the peptide core and reacts with both the plasma and cellular forms of fibronectin. It blocks the fibronectin-medicated cell attachment not by disrupting the collagen-fibronectin interaction, but by interfering with the attachment of fibronectin to its receptor on the cell surface.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34785)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Fibronectin Antibody (SPM539)
Immunohistochemistry-Paraffin: Fibronectin Antibody (SPM539) [NBP2-32848]
Immunohistochemistry-Paraffin: Fibronectin Antibody (SPM539) [NBP2-32848] - Formalin-fixed, paraffin-embedded human Pancreatic Adeno Carcinoma stained with Fibronectin Monoclonal Antibody (SPM539).Applications for Fibronectin Antibody (SPM539)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Fibronectin
Additional Fibronectin Products
Product Documents for Fibronectin Antibody (SPM539)
Product Specific Notices for Fibronectin Antibody (SPM539)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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