Fibronectin Antibody [DyLight 405]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91258V

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NBP1-91258AF350

Alexa Fluor 405

NBP1-91258AF405

Alexa Fluor 488

NBP1-91258AF488

Alexa Fluor 532

NBP1-91258AF532

Alexa Fluor 594

NBP1-91258AF594

Alexa Fluor 647

NBP1-91258AF647

Alexa Fluor 700

NBP1-91258AF700

Alexa Fluor 750

NBP1-91258AF750

Allophycocyanin

NBP1-91258APC

Biotin

NBP1-91258B

CoraFluor 1

NBP1-91258CL1

DyLight 350

NBP1-91258UV

DyLight 488

NBP1-91258G

DyLight 550

NBP1-91258R

DyLight 594

NBP1-91258DL594

DyLight 650

NBP1-91258C

DyLight 680

NBP1-91258FR

DyLight 755

NBP1-91258IR

FITC

NBP1-91258F

HRP

NBP1-91258H

Janelia Fluor 525

NBP1-91258JF525

Janelia Fluor 549

NBP1-91258JF549

Janelia Fluor 585

NBP1-91258JF585

Janelia Fluor 635

NBP1-91258JF635

Janelia Fluor 646

NBP1-91258JF646

Janelia Fluor 669

NBP1-91258JF669

mFluor Violet 450 SE

NBP1-91258MFV450

mFluor Violet 500 SE

NBP1-91258MFV500

mFluor Violet 610 SE

NBP1-91258MFV610

PE

NBP1-91258PE

PerCP

NBP1-91258PCP

Forumulation

Catalog #

BSA Free

NBP1-91258

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Bovine, Canine, Equine, Feline

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

DyLight 405 (Excitation = 400 nm, Emission = 420 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Fibronectin Antibodies »

Product Specifications

Immunogen

A synthetic peptide made toward the C-terminal region of the human Fibronectin protein (canonical isoform within residues 2300-2380).

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 29439094). Equine reactivity reported in scientific literature (PMID: 30450188). Rat reactivity reported in scientific literature (PMID: 30699330).

Localization

Secreted, extracellular space, extracellular matrix.

Marker

Mesenchymal Cells Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for Fibronectin Antibody [DyLight 405]

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Simple Western

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Fibronectin

Fibronectin EDA (FN-EDA, FN, fibronectin 1) is a glycoprotein that is involved in cell adhesion as well as cell migration processes such as embryogenesis, wound healing, host defense and metastasis. Fibronectin appears to be an important factor in the ability of cancerous cells to metastasize by inducing matrix metalloproteinases (MMPs). In a study of human laryngeal carcinoma cells, researchers showed that FN-induced expression of MMP-9 increased the DNA binding activity of transcription factors NF-kappa-B, Activator protein-1 and Specificity protein-1 to the promoter region (PMID: 20638438). Additionally, a study of renal cell cancer showed that the mRNA expression of fibronectin 1 was higher in patients with renal cell cancer as compared to patients with healthy kidneys. This disparity in mRNA expression levels could potentially be used to determine the aggressiveness of renal cell cancer as well as indicate the progression of the disease in patients (PMID: 20860816). Furthermore, a knockdown study of endogenous FN in the A431-III cell line showed a significant decrease of the migratory and invasive activity of the cells as well as an inhibition of secreted MMP-9 (PMID: 21036738).

Alternate Names

CIG, ED-B, FINC, FN1, FNZ, GFND2, LETS, SMDCF

Gene Symbol

FN1

Additional Fibronectin Products

Product Documents for Fibronectin Antibody [DyLight 405]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for Fibronectin Antibody [DyLight 405]

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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