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Ficolin-1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84706

Novus Biologicals, part of Bio-Techne
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NBP1-84706
NBP1-84706-25ul

Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LAVLLVLFLHIKNLPAQAADTCPEVKVVGLEGSDKLTILRGCPGLPGAPGPKGEAGVIGERGERGLPGAPGKAGPVGPKGDRGEKGMRGEKGDAGQSQSCATGPRNCKD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Ficolin-1 Antibody

Immunohistochemistry-Paraffin: Ficolin-1 Antibody [NBP1-84706]

Immunohistochemistry-Paraffin: Ficolin-1 Antibody [NBP1-84706]

Immunohistochemistry-Paraffin: Ficolin-1 Antibody [NBP1-84706] - Analysis in human bone marrow and pancreas tissues using NBP1-84706 antibody. Corresponding Ficolin-1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Ficolin-1 Antibody [NBP1-84706]

Immunohistochemistry-Paraffin: Ficolin-1 Antibody [NBP1-84706]

Immunohistochemistry-Paraffin: Ficolin-1 Antibody [NBP1-84706] - Staining of human bone marrow, gastrointestinal, lymphoid tissues and pancreas using Anti-Ficolin-1 antibody NBP1-84706 (A) shows similar protein distribution across tissues to independent antibody NBP1-84705 (B).
Western Blot: Ficolin-1 Antibody [NBP1-84706]

Western Blot: Ficolin-1 Antibody [NBP1-84706]

Western Blot: Ficolin-1 Antibody [NBP1-84706] - Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 18 Lane 2: Human cell line RT-4 Lane 3: Human cell line U-251MG sp Lane 4: Human cell line A-431 Lane 5: Human liver tissue

Applications for Ficolin-1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ficolin-1

M-Ficolin (Ficolin-1) is a non-serum protein that is expressed in leukocytes and lung. M-Ficolin precipitates with mannose-binding lectin (MBL)-associated serine proteases (MASP)-1 and MASP-2, indicating that M-Ficolin forms complexes with MASP-1 and MASP-2, recognizes certain types of bacteria and subsequently activates the lectin pathway (1).

Long Name

Ficolin [Collagen/Fibrinogen Domain Containing] 1

Alternate Names

FCN1, Fcna, FCNM, Ficolin1

Gene Symbol

FCN1

Additional Ficolin-1 Products

Product Documents for Ficolin-1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Ficolin-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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