Filaggrin Antibody (rFLG/1945) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14280
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 kappa Clone # rFLG/1945
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for Filaggrin Antibody (rFLG/1945) - Azide and BSA Free
Immunogen
Recombinant human Filaggrin protein fragment (around aa 198-288) (exact sequence is proprietary) (Uniprot: P20930 )
Localization
Cytoplasm
Marker
Keratinocyte Differentiation Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13892).
Antibody without azide - store at -20 to -80C. Antibody with azide - store at 2 to 8C. Non-hazardous.
Theoretical molecular weight: 26-45kDa (Processed); 350kDa (Profilaggrin)
Antibody without azide - store at -20 to -80C. Antibody with azide - store at 2 to 8C. Non-hazardous.
Theoretical molecular weight: 26-45kDa (Processed); 350kDa (Profilaggrin)
Applications for Filaggrin Antibody (rFLG/1945) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Filaggrin
Alternate Names
ATOD2, epidermal filaggrin, filaggrin, Profilaggrin
Gene Symbol
FLG
Additional Filaggrin Products
Product Documents for Filaggrin Antibody (rFLG/1945) - Azide and BSA Free
Product Specific Notices for Filaggrin Antibody (rFLG/1945) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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