Filaggrin Antibody (rFLG/1945)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13892
Recombinant Monoclonal Antibody
![Immunohistochemistry-Paraffin: Filaggrin Antibody (rFLG/1945) [NBP3-13892]](https://resources.bio-techne.com/images/products/Filaggrin-Antibody-rFLG-1945-Immunohistochemistry-Paraffin-NBP3-13892-img0001.jpg "Immunohistochemistry-Paraffin: Filaggrin Antibody (rFLG/1945) [NBP3-13892]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 kappa Clone # rFLG/1945
Concentration
0.2 mg/ml
Product Summary for Filaggrin Antibody (rFLG/1945)
Immunogen
Recombinant human Filaggrin protein fragment (around aa 198-288) (exact sequence is proprietary) (Uniprot: P20930 )
Localization
Cytoplasm
Marker
Keratinocyte Differentiation Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14280)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Non-hazardous.
Theoretical molecular weight: 26-45kDa (Processed); 350kDa (Profilaggrin)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Non-hazardous.
Theoretical molecular weight: 26-45kDa (Processed); 350kDa (Profilaggrin)
Scientific Data Images for Filaggrin Antibody (rFLG/1945)
Immunohistochemistry-Paraffin: Filaggrin Antibody (rFLG/1945) [NBP3-13892]
Immunohistochemistry-Paraffin: Filaggrin Antibody (rFLG/1945) [NBP3-13892] - Formalin-fixed, paraffin-embedded human skin stained with Filaggrin antibody (rFLG/1945).Applications for Filaggrin Antibody (rFLG/1945)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Filaggrin
Alternate Names
ATOD2, epidermal filaggrin, filaggrin, Profilaggrin
Gene Symbol
FLG
UniProt
Additional Filaggrin Products
Product Documents for Filaggrin Antibody (rFLG/1945)
Product Specific Notices for Filaggrin Antibody (rFLG/1945)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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