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Filaggrin Antibody (rFLG/1945)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13892

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 kappa Clone # rFLG/1945

Concentration

0.2 mg/ml

Product Specifications

Immunogen

Recombinant human Filaggrin protein fragment (around aa 198-288) (exact sequence is proprietary) (Uniprot: P20930 )

Localization

Cytoplasm

Marker

Keratinocyte Differentiation Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14280)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Non-hazardous.

Theoretical molecular weight: 26-45kDa (Processed); 350kDa (Profilaggrin)

Scientific Data Images for Filaggrin Antibody (rFLG/1945)

Immunohistochemistry-Paraffin: Filaggrin Antibody (rFLG/1945) [NBP3-13892]

Immunohistochemistry-Paraffin: Filaggrin Antibody (rFLG/1945) [NBP3-13892]

Immunohistochemistry-Paraffin: Filaggrin Antibody (rFLG/1945) [NBP3-13892] - Formalin-fixed, paraffin-embedded human skin stained with Filaggrin antibody (rFLG/1945).

Applications for Filaggrin Antibody (rFLG/1945)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Filaggrin

Alternate Names

ATOD2, epidermal filaggrin, filaggrin, Profilaggrin

Gene Symbol

FLG

UniProt

Additional Filaggrin Products

Product Documents for Filaggrin Antibody (rFLG/1945)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Filaggrin Antibody (rFLG/1945)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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