Filaggrin Antibody (SPM181) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54365
![Immunohistochemistry-Paraffin: Filaggrin Antibody (SPM181) - Azide and BSA Free [NBP2-54365]](https://resources.bio-techne.com/images/products/Filaggrin-Antibody-SPM181-Immunohistochemistry-Paraffin-NBP2-54365-img0001.jpg "Immunohistochemistry-Paraffin: Filaggrin Antibody (SPM181) - Azide and BSA Free [NBP2-54365]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM181
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for Filaggrin Antibody (SPM181) - Azide and BSA Free
Immunogen
Recombinant human Filaggrin protein (Uniprot: P20930)
Localization
Cytoplasmic
Marker
Keratinocyte Differentiation Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53244).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Filaggrin Antibody (SPM181) - Azide and BSA Free
Immunohistochemistry-Paraffin: Filaggrin Antibody (SPM181) - Azide and BSA Free [NBP2-54365]
Immunohistochemistry-Paraffin: Filaggrin Antibody (SPM181) [NBP2-54365] - Formalin-fixed, paraffin-embedded human Skin stained with Filaggrin Monoclonal Antibody (SPM181).Applications for Filaggrin Antibody (SPM181) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Filaggrin
Alternate Names
ATOD2, epidermal filaggrin, filaggrin, Profilaggrin
Gene Symbol
FLG
Additional Filaggrin Products
Product Documents for Filaggrin Antibody (SPM181) - Azide and BSA Free
Product Specific Notices for Filaggrin Antibody (SPM181) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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