Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM181

Concentration

0.2 mg/ml

View all Filaggrin Antibodies »

Product Summary for Filaggrin Antibody (SPM181)

Immunogen

Recombinant human Filaggrin protein (Uniprot: P20930)

Localization

Cytoplasmic

Marker

Keratinocyte Differentiation Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54365)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Filaggrin Antibody (SPM181)

Immunohistochemistry-Paraffin: Filaggrin Antibody (SPM181) [NBP2-53244]

Immunohistochemistry-Paraffin: Filaggrin Antibody (SPM181) [NBP2-53244]

Immunohistochemistry-Paraffin: Filaggrin Antibody (SPM181) [NBP2-53244] - Formalin-fixed, paraffin-embedded human Skin stained with Filaggrin Monoclonal Antibody (SPM181).

Applications for Filaggrin Antibody (SPM181)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Filaggrin

Alternate Names

ATOD2, epidermal filaggrin, filaggrin, Profilaggrin

Gene Symbol

FLG

UniProt

P20930 (Human)

Additional Filaggrin Products

Product Documents for Filaggrin Antibody (SPM181)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Filaggrin Antibody (SPM181)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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