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Filensin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91902

Novus Biologicals, part of Bio-Techne
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NBP1-91902
NBP1-91902-25ul

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SAHECHDDEIQLYNEQIETLRKEIEETERVLEKSSYDCRQLAVAQQTLKNELDRYHRIIEIEGNRLTSAFIETPIPLFTQSHGVSLSTGSGGKD

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (82%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Filensin Antibody

Immunocytochemistry/ Immunofluorescence: Filensin Antibody [NBP1-91902]

Immunocytochemistry/ Immunofluorescence: Filensin Antibody [NBP1-91902]

Immunocytochemistry/Immunofluorescence: Filensin Antibody [NBP1-91902] - Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.
Immunohistochemistry-Paraffin: Filensin Antibody [NBP1-91902]

Immunohistochemistry-Paraffin: Filensin Antibody [NBP1-91902]

Immunohistochemistry-Paraffin: Filensin Antibody [NBP1-91902] - Staining of human kidney.
Immunohistochemistry-Paraffin: Filensin Antibody [NBP1-91902]

Immunohistochemistry-Paraffin: Filensin Antibody [NBP1-91902]

Immunohistochemistry-Paraffin: Filensin Antibody [NBP1-91902] - Staining of human eye shows moderate cytoplasmic positivity in the lens.

Applications for Filensin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:5000 - 1:10000

Immunohistochemistry-Paraffin

1:5000 - 1:10000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Filensin

More than 99% of the vertebrate ocular lens is comprised of terminally differentiated lens fiber cells. Two lens-specific intermediate filament-like proteins, CP49 (also known as phakinin) and filensin (or CP115), are expressed only after fiber cell differentiation has begun. Both proteins are found in a structurally unique cytoskeletal element that is referred to as the beaded filament (BF).

Alternate Names

Beaded filament structural protein 1, beaded filament structural protein 1, filensin, CP115Lens intermediate filament-like heavy, filensin, Lens fiber cell beaded-filament structural protein CP 115, LIFL-H115 KD

Gene Symbol

BFSP1

Additional Filensin Products

Product Documents for Filensin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Filensin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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