FIP1/RCP Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37698
![Western Blot: FIP1/RCP Antibody [NBP2-37698]](https://resources.bio-techne.com/images/products/FIP1-RCP-Antibody-Western-Blot-NBP2-37698-img0001.jpg "Western Blot: FIP1/RCP Antibody [NBP2-37698]")
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
This antibody maps to a region between residue 675 to 725 of human RAB11 Coupling Protein using the numbering given in entry NP_001002814.2 (GeneID 80223).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for FIP1/RCP Antibody
Western Blot: FIP1/RCP Antibody [NBP2-37698]
Western Blot: FIP1/RCP Antibody [NBP2-37698] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Affinity purified rabbit anti-RCP antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Immunoprecipitation: FIP1/RCP Antibody [NBP2-37698]](https://resources.bio-techne.com/images/products/FIP1-RCP-Antibody-Immunoprecipitation-NBP2-37698-img0002.jpg "Immunoprecipitation: FIP1/RCP Antibody [NBP2-37698]")
Immunoprecipitation: FIP1/RCP Antibody [NBP2-37698]
Immunoprecipitation: FIP1/RCP Antibody [NBP2-37698] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Affinity purified rabbit anti-RCP antibody used for IP at 6 ug per reaction. RCP was also immunoprecipitated by rabbit anti-RCP antibody. For blotting immunoprecipitated RCP, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for FIP1/RCP Antibody
Application
Recommended Usage
Immunoprecipitation
2 - 10 ug/mg lysate
Western Blot
1:2000 - 1:10000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: FIP1/RCP
Alternate Names
DKFZp686E2214, EC 3.2.1.28, EC 6.1.1.7, FLJ22524, FLJ22622, MGC78448, NOEL1A, RAB11 coupling protein, RAB11 family interacting protein 1 (class I), rab11 family-interacting protein 1, rab11-FIP1, Rab-coupling protein, Rab-interacting recycling protein, RCPRab effector protein
Gene Symbol
RAB11FIP1
UniProt
Additional FIP1/RCP Products
Product Documents for FIP1/RCP Antibody
Product Specific Notices for FIP1/RCP Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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