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FLAD1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84752

Novus Biologicals, part of Bio-Techne
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NBP1-84752
NBP1-84752-25ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for FLAD1 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TNTFFLCRTLRSLGVQVCRVSVVPDEVATIAAEVTSFSNRFTHVLTAGGIGPTHDDVTFEAVAQAFGDELKPHPKLEAATKA

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (89%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FLAD1 Antibody

Western Blot: FLAD1 Antibody [NBP1-84752]

Western Blot: FLAD1 Antibody [NBP1-84752]

Western Blot: FLAD1 Antibody [NBP1-84752] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/ Immunofluorescence: FLAD1 Antibody [NBP1-84752]

Immunocytochemistry/ Immunofluorescence: FLAD1 Antibody [NBP1-84752]

Immunocytochemistry/Immunofluorescence: FLAD1 Antibody [NBP1-84752] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
Immunohistochemistry-Paraffin: FLAD1 Antibody [NBP1-84752]

Immunohistochemistry-Paraffin: FLAD1 Antibody [NBP1-84752]

Immunohistochemistry-Paraffin: FLAD1 Antibody [NBP1-84752] - Staining of human duodenum shows cytoplasmic positivity in glandular cells.

Applications for FLAD1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FLAD1

The FLAD1 gene encodes the enzyme that catalyzes adenylation of flavin mononucleotide (FMN) to form flavin adeninedinucleotide (FAD) coenzyme. Alternatively spliced transcript variants encoding distinct isoforms have been observed.(provided by RefSeq)

Alternate Names

EC 2.7.7, FAD1, FAD1 flavin adenine dinucleotide synthetase homolog (S. cerevisiae), Fad1, flavin adenine dinucleotide synthetase, homolog (yeast), FAD-synthetase, Flavin adenine dinucleotide synthase, flavin adenine dinucleotide synthetase, flavin adenine dinucleotide synthetase, homolog, MGC31803, MGC40255, PP591

Gene Symbol

FLAD1

Additional FLAD1 Products

Product Documents for FLAD1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FLAD1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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