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FLI1 Antibody (FLI1/1312)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-53335

Novus Biologicals, part of Bio-Techne
Catalog #
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NBP2-53335-20ug
NBP2-53335-100ug

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # FLI1/1312

Concentration

0.2 mg/ml

Product Specifications

Immunogen

Recombinant fragment (aa 107-261) of human FLI1 protein with hexa-histidine tag (Uniprot: Q01543)

Localization

Nuclear

Marker

Ewing's Sarcoma & Endothelial Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from rabbit anti-serum by Protein A. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP2-54423)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for FLI1 Antibody (FLI1/1312)

Western Blot: FLI1 Antibody (FLI1/1312) [NBP2-53335]

Western Blot: FLI1 Antibody (FLI1/1312) [NBP2-53335]

Western Blot: FLI1 Antibody (FLI1/1312) [NBP2-53335] - Western Blot of THP1 and Raji Cell Lysate using FLI1 Monoclonal Antibody (FLI1/1312)

Applications for FLI1 Antibody (FLI1/1312)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
Western Blot: 1-2ug/ml for 60 minutes at RT.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: FLI1

Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. This family of genes currently includes Ets-1, Ets-2 Erg-1, Erg-2, Elk, E74, Fli-1, PU.1 and PEA3. Members of the Ets gene family exhibit varied pattern of tissue expression, and share a highly conserved carboxy terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence.

Long Name

Friend Leukemia Integration 1

Alternate Names

ERGB, SIC-1

Gene Symbol

FLI1

UniProt

Additional FLI1 Products

Product Documents for FLI1 Antibody (FLI1/1312)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FLI1 Antibody (FLI1/1312)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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