FLIP Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77020

Novus Biologicals, part of Bio-Techne
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NBP1-77020
NBP1-77020-0.025mg
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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all FLIP Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 17 amino acid peptide near the carboxy terminus of mouse FLIP. The immunogen is located within the last 50 amino acids of FLIP. Amino Acid Squence: DKVYAWNSGVSSKEKYS

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 15090542)

Specificity

FLIP has short form (FLIPS) and long form (FLIPL) and antibody recognizes the FLIPL only.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FLIP Antibody - BSA Free

Western Blot: FLIP AntibodyBSA Free [NBP1-77020]

Western Blot: FLIP AntibodyBSA Free [NBP1-77020]

Western Blot: FLIP Antibody [NBP1-77020] - Analysis of FLIP in K562 cell lysate with FLIP antibody at (A) 1 and (B) 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: FLIP Antibody - BSA Free [NBP1-77020]

Immunocytochemistry/ Immunofluorescence: FLIP Antibody - BSA Free [NBP1-77020]

Immunocytochemistry/Immunofluorescence: FLIP Antibody [NBP1-77020] - 3T3 cells with FLIP antibody at 5 ug/ml.
Western Blot: FLIP AntibodyBSA Free [NBP1-77020]

Western Blot: FLIP AntibodyBSA Free [NBP1-77020]

Western Blot: FLIP Antibody [NBP1-77020] - Western blot analysis of FLIP in NIH/3T3 whole cell lysate with FLIP antibody at 1:500 dilution.

Applications for FLIP Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

5 ug/ml

Western Blot

1-2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Ion exchange chromatography

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FLIP

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) containing adapter molecules and members of the ICE/CED-3 protease family.Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP respectively. The human FLIP was also cloned by several labs independently and termed Casper , I-FLICE, FLAME-1, CASH, CLARP and MRIT. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by all known death receptors CD95, DR3, TRAIL-R and TNFR1.

Long Name

FLICE Inhibitory Proteins

Alternate Names

CASPER, CFLAR, FLAME-1, I-FLICE

Gene Symbol

CFLAR

UniProt

NP_997536 (Mouse)

Additional FLIP Products

Product Documents for FLIP Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for FLIP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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