Flt-3/Flk-2/CD135 Antibody (7E8.2C8) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80739

Novus Biologicals, part of Bio-Techne
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NBP2-80739
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BSA Free
NBP2-42210
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Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Lambda Clone # 7E8.2C8

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Flt-3/Flk-2 Antibodies »

Product Summary for Flt-3/Flk-2/CD135 Antibody (7E8.2C8) - Azide and BSA Free

Immunogen

Partial recombinant human FLT3 protein (between amino acids 250-500) [UniProt P36888]

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: ~86% to Mouse and Rat's FLT3 protein.

Localization

Endoplasmic reticulum lumen, Membrane

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Lambda

Scientific Data Images for Flt-3/Flk-2/CD135 Antibody (7E8.2C8) - Azide and BSA Free

Western Blot: Flt-3/Flk-2/CD135 Antibody (7E8.2C8)Azide and BSA Free [NBP2-80739]

Western Blot: Flt-3/Flk-2/CD135 Antibody (7E8.2C8)Azide and BSA Free [NBP2-80739]

Western Blot: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) - Azide and BSA Free [NBP2-80739] - WB analysis of lysates from (A) Human Embryonic Kidney 293 / HEK293 and (B) Human Embryonic carcinoma NCCIT cell lines using Flt-3/Flk-2/CD135 antibody (clone 7E8.2C8) at 2ug/ml concentration. The antibody detected single specific bands in both samples at the expected molecular weight position. Image from the standard format of this antibody.
Immunocytochemistry: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) - Azide and BSA Free [NBP2-80739]

Immunocytochemistry: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) - Azide and BSA Free [NBP2-80739]

Immunocytochemistry: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) - Azide and BSA Free [NBP2-80739] - IHC analysis of a formalin fixed paraffin embedded tissue section of human liver cancer using Flt-3/Flk-2/CD135 antibody (clone 7E2.2C8) at 15 ug/ml concentration. The antibody generated specific cytoplasmic immunostaining of FLT3 in the cancerous cells w
Flow Cytometry: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) - Azide and BSA Free [NBP2-80739]

Flow Cytometry: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) - Azide and BSA Free [NBP2-80739]

Flow Cytometry: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) - Azide and BSA Free [NBP2-80739] - HEK293 cells were stained (surface) with Flt-3/Flk-2/CD135 antibody (clone: 7E8.2C8; red) or isotype control (mouse IgG2 lambda; blue) and positive staining observed using PE conjugated mouse anti-IgG(H+L) secondary antibody. Live cells (PPI negative) were gated for analysis Image from the standard format of this antibody.

Applications for Flt-3/Flk-2/CD135 Antibody (7E8.2C8) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

2 ug/million cells

Immunohistochemistry

5-15 ug/ml

Immunohistochemistry-Paraffin

5-15 ug/ml

Western Blot

2 ug/ml
Application Notes
FLT3 is a 993 amino acids long protein with predicted molecular weight of 112.9 kDa, however, it undergoes extensive glycosylation and phosphorylation so that the mature protein will have relatively higher molecular weights. In WB assay, the glycosylated protein species may run between 113-160 kDa position, while the phosphorylated protein may result on generation of a doublet band.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Flt-3/Flk-2

CD135 is a tyrosine kinase receptor expressed on normal cells including CD34+ hematopoietic stem cells, myelomonocytic progenitors, primitive B cell progenitors, and thymocytes. CD135 is also expressed on malignant hematopoietic cells including AML, ALL and CML BC. CD135, also known as FMS-like tyrosine kinase 3, FLT3, STK1, and Flk2, is a growth factor receptor that binds the FLT3 ligand to promote the growth and differentiation of primitive hematopoietic cells. The intracytoplasmic domain of CD135 is modified by phosphorylation and has been shown to interact with Grb2, SOCS1, VAV1, and Shc. In humans, expression of Flt3 is restricted to subsets of CD34 positive as well as CD34 negative normal bone marrow cells. In these cells, the level of expression of Flt3 is rather low. Most of the CD34 bright Flt3+ cells co-express CD117 at high levels. They may represent early cycling, but not quiescent stem cells. Flt3+ cells in the CD34lo and CD34- populations do not co-express CD117 molecule and may represent B lymphoid precursors.

Long Name

fms-like Tyrosine Kinase 3

Alternate Names

CD135, Flk-2, Flt3, STK-1

Gene Symbol

FLT3

Additional Flt-3/Flk-2 Products

Product Documents for Flt-3/Flk-2/CD135 Antibody (7E8.2C8) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Flt-3/Flk-2/CD135 Antibody (7E8.2C8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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