Fluorescein Antibody [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2082
Key Product Details
Species Reactivity
Non-species specific
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Fluorescein conjugated to Goat IgG
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room
Fluorescein Antibody Biotin Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein coupled to sepharose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum and Fluorescein conjugated IgG.
Fluorescein Antibody Biotin Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein coupled to sepharose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum and Fluorescein conjugated IgG.
Scientific Data Images for Fluorescein Antibody [Biotin]
Western Blot: Fluorescein Antibody [Biotin] [NB100-2082] - Lane 1: BSA conjugated Fluorescein. Lane 2: None. Load: 50 ng per lane. Primary antibody: Fluorescein antibody at 1:1000 for 60 min at RT. Secondary antibody: Peroxidase conjugated Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 70 kDa, 70 kDa for BSA-FITC. Other band(s): None.
Applications for Fluorescein Antibody [Biotin]
Application
Recommended Usage
ELISA
1:10000-1:50000
Immunohistochemistry
1:1000-1:5000
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:2000-1:10000
Application Notes
This product has been tested by ELISA, dot blot and western blot and is suitable for immunoblotting, ELISA, in situ hybridization, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Fluorescein
Alternate Names
FITC, fluorescein isothyocianate
Additional Fluorescein Products
Product Documents for Fluorescein Antibody [Biotin]
Product Specific Notices for Fluorescein Antibody [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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