Fluorescein Antibody [Texas Red]
Novus Biologicals, part of Bio-Techne | Catalog # NB110-3648
Key Product Details
Species Reactivity
Non-species specific
Applications
Validated:
Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot
Cited:
Immunohistochemistry-Frozen
Label
Texas Red (Excitation = 589 nm, Emission = 615 nm)
Antibody Source
Polyclonal Goat IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Fluorescein conjugated to Goat IgG
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room
Fluorescein Antibody Texas Red(TM) Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Fluorescein conjugated Bovine Serum Albumin.
Fluorescein Antibody Texas Red(TM) Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Fluorescein conjugated Bovine Serum Albumin.
Applications for Fluorescein Antibody [Texas Red]
Application
Recommended Usage
Fluorophore-linked immunosorbent assay
>1:20000
Immunocytochemistry/ Immunofluorescence
>1:20000
Western Blot
>1:10000
Application Notes
This product is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Use in Immunohistochemistry-Frozen reported in scientific literature (PMID:19667884)
Use in Immunohistochemistry-Frozen reported in scientific literature (PMID:19667884)
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Fluorescein
Alternate Names
FITC, fluorescein isothyocianate
Additional Fluorescein Products
Product Documents for Fluorescein Antibody [Texas Red]
Product Specific Notices for Fluorescein Antibody [Texas Red]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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