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FOLR1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB600-865

Novus Biologicals, part of Bio-Techne
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NB600-865-100ug

Key Product Details

Species Reactivity

Bovine

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

FOLR1 [Bovine Milk] (Uniprot: P02702)

Reactivity Notes

Cross reactivity against FOLR1 from other tissues and species may occur but have not been specifically determined.

Localization

Attached to the membrane by a GPI-anchor

Specificity

Cross reactivity against FOLR1 from other tissues and species may occur but have not been specifically determined.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Folate Binding Protein [Bovine Milk]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for FOLR1 Antibody

Western Blot: FOLR1 Antibody [NB600-865]

Western Blot: FOLR1 Antibody [NB600-865]

Western Blot: FOLR1 Antibody [NB600-865] - Analysis using the Biotin conjugate of NB600-865. Detection of Lane 1: Blotto. Load: 50.0 ug per lane. Primary antibody: Goat anti-Folate Binding Protein (Bovine Milk) Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: HRP Streptav

Applications for FOLR1 Antibody

Application
Recommended Usage

ELISA

1:5000-1:20000

Immunoprecipitation

1:100

Western Blot

1:500-1:5000
Application Notes
This product has been assayed against 1.0 ug of Folate Binding Protein [Bovine Milk] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:40,000 of the reconstitution concentration is suggested for this product. Highest levels of antigen binding and antibody reactivity were observed using NuncO brand MaxiSorp immunoplates.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Background: FOLR1

Folate binding protein, has a high affinity for folic acid and for several reduced folic acid derivatives and mediates delivery of 5 methyltetrahydrofolate to the interior of cells. Membrane bound and soluble forms of a high affinity folate binding protein have been found in kidney, placenta, serum, milk, and in several cell lines. The 2 forms have similar binding characteristics for folates, are immunologically crossreactive, and, based upon limited amino acid sequence data, are nearly identical. There may be a precursor product relationship between the membrane and soluble forms, the membrane form having additional amino acid residues and greater molecular weight. The membrane form has been shown to mediate the transport of folate in cells grown in physiologic concentrations of folate. A function for the soluble form, which is found in serum, milk, and urine, has not been identified.

Long Name

Folate Receptor 1

Alternate Names

FBP, Folbp1, FR-alpha, MOv18

Gene Symbol

FOLR1

UniProt

Additional FOLR1 Products

Product Documents for FOLR1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FOLR1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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