FOLR1 Antibody [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77706
Key Product Details
Species Reactivity
Bovine
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
FOLR1 [Bovine Milk] (Uniprot: P02702)
Reactivity Notes
Cross reactivity against FOLR1 from other tissues and species may occur but have not been specifically determined.
Localization
Attached to the membrane by a GPI-anchor
Specificity
Cross reactivity against FOLR1 from other tissues and species may occur but have not been specifically determined.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Folate Binding Protein [Bovine Milk]
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Folate Binding Protein [Bovine Milk]
Scientific Data Images for FOLR1 Antibody [Biotin]
Western Blot: FOLR1 Antibody [Biotin] [NBP1-77706] - Lane 1: Blotto. Load: 50.0 ug per lane. Primary antibody: Goat anti-Folate Binding Protein (Bovine Milk) Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 minutes at RT. Predicted/Observed size: 28 kDa, 30 kDa for Folate Binding Protein. Migrates slightly higher. Other band(s): non-specific proteins in Blotto.
Applications for FOLR1 Antibody [Biotin]
Application
Recommended Usage
ELISA
1:1000-1:2500
Immunoprecipitation
1:100
Western Blot
1:500-1:1000
Application Notes
This product has been tested by western blot and is suitable to be assayed against 1.0 ug of Folate Binding Protein in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: FOLR1
Long Name
Folate Receptor 1
Alternate Names
FBP, Folbp1, FR-alpha, MOv18
Gene Symbol
FOLR1
UniProt
Additional FOLR1 Products
Product Documents for FOLR1 Antibody [Biotin]
Product Specific Notices for FOLR1 Antibody [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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