FoxC2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1269
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Predicted:
Bovine (100%), Canine (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
Flow Cytometry, Peptide ELISA, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence RHAAPYSYDCTKY corresponding to C-Terminus according to NP_005242.1.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for FoxC2 Antibody
![Western Blot: FoxC2 Antibody [NB100-1269]](https://resources.bio-techne.com/images/products/FoxC2-Antibody-Western-Blot-NB100-1269-img0012.jpg "Western Blot: FoxC2 Antibody [NB100-1269]")
Western Blot: FoxC2 Antibody [NB100-1269]
Western Blot: FoxC2 Antibody [NB100-1269] - Staining of nuclear HEK293 cell lysate with antibody at 2 ug/mL (A) and negative control human pancreas cell lysate with antibody at 1 ug/mL (B) (RIPA buffer 35 ug total protein per lane). Detected by chemiluminescence.![Flow Cytometry: FoxC2 Antibody [NB100-1269]](https://resources.bio-techne.com/images/products/FoxC2-Antibody-Flow-Cytometry-NB100-1269-img0011.jpg "Flow Cytometry: FoxC2 Antibody [NB100-1269]")
Flow Cytometry: FoxC2 Antibody [NB100-1269]
Flow Cytometry: FoxC2 Antibody [NB100-1269] - Analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.Applications for FoxC2 Antibody
Application
Recommended Usage
Flow Cytometry
10 ug/mL
Peptide ELISA
Detection limit 1:4000
Western Blot
2 ug/mL
Application Notes
IHC validated for previous lots. Use in IHC-frozen, and ICC/IF reported in scientific literature (PMID: 23862012).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4.5 using NB100-1269 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: FoxC2
Long Name
Forkhead Box C2
Alternate Names
Fkh14, LD, MFH1
Gene Symbol
FOXC2
UniProt
Additional FoxC2 Products
Product Documents for FoxC2 Antibody
Product Specific Notices for FoxC2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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