FOXG1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1099
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Flow Cytometry, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-HQNQGSSSNPLIH corresponding to C-Terminus according to NP_005240.3.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for FOXG1 Antibody
Western Blot: FOXG1 Antibody [NB100-1099] -
Western Blot: FOXG1 Antibody [NB100-1099] - (1ug/ml) staining of U251 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Western Blot: FOXG1 Antibody [NB100-1099] -
Western Blot: FOXG1 Antibody [NB100-1099] - (0.5ug/ml) staining of Human Cerebellum (A) and Cerebral Cortex (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Flow Cytometry: FOXG1 Antibody [NB100-1099] -
Flow Cytometry: FOXG1 Antibody [NB100-1099] - Flow cytometric analysis of paraformaldehyde fixed U251 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.Applications for FOXG1 Antibody
Application
Recommended Usage
Flow Cytometry
10 ug/ml
Peptide ELISA
Detection limit 1:64000
Western Blot
0.3 - 1 ug/ml
Application Notes
WB: Approx. 48kDa band observed in lysates of cell line U251, and approx. 48-50kDa in Human Cerebellum and Cerebral Cortex lysates (calculated MW of 52.4kDa according to NP_005240.3 (FOXG1B), and 50.5kDa according to CAA55038.1 (FOXG1A). An additional band at 70-75kDa was observed in some lysates and was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: FOXG1
Alternate Names
BF-1, BF1HBF-2, BF2, BF-2, Brain factor 1, Brain factor 2, FHKL3, FKHL1forkhead-like 1, FKHL2, FKHL3, FKHL4, forkhead box G1, forkhead box G1A, forkhead box G1B, forkhead box G1C, Forkhead box protein G1A, Forkhead box protein G1B, Forkhead box protein G1C, forkhead-like 3, forkhead-like 4, Forkhead-related protein FKHL1, Forkhead-related protein FKHL2, Forkhead-related protein FKHL3, FOXG1A, FOXG1Bforkhead-like 2, FOXG1C, HBF-1, hBF-2, HBF-3, HFK1HBF-G2, HFK2HBF2, HFK3KHL2, oncogene QIN, QINFKH2forkhead box protein G1
Gene Symbol
FOXG1
Additional FOXG1 Products
Product Documents for FOXG1 Antibody
Product Specific Notices for FOXG1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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