Western Blot: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis of (A) Ntera2 cell lysate, (B) HEK293 cell lysate, and (C) human placenta tissue using FOXO1A antibody at a concentration of 2 ug/ml.

Immunocytochemistry/ Immunofluorescence: FoxO1/FKHR Antibody (83N7F8) - BSA Free [NBP2-31376]

Immunocytochemistry/Immunofluorescence: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - FOXO1A antibody was tested in Ntera2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). An antibody concentration of 0.1 ug/ml was used. Image objective 40x.

Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (83N7F8) - BSA Free [NBP2-31376]

Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis of FOXO1A protein in a section of normal human uterus by using FOXO1A antibody at a concentration of 5 ug/ml. The columnar cells of straight non-convoluted glands in uterous showed specific staining for FOXO1A in the cellular cytoplasm as well as nuclei, whereas the stromal cells showed mild cytoplasmic staining only.

Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) - BSA Free [NBP2-31376]

Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - An intracellular stain was performed on RH-30 cells with FoxO1/FKHR Antibody [83N7F8] NBP2-31376AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Western Blot: FoxO1/FKHR Antibody (83N7F8)BSA Free [NBP2-31376]

Western Blot: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Detection of FOXO1A / FOXO1 protein in (A) Hela cells lysate, (B) Jurkat cell lysate, and (C) partial recombinant protein by using FOXO1A antibody at a concentration of 0.5 ug/ml for lysate and 0.05 ug/ml for the recombinant protein.

Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (83N7F8) - BSA Free [NBP2-31376]

Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis of FOXO1A protein in a section of normal human kidney by using FOXO1A antibody at a concentration of 5 ug/ml. Cytoplasmic and nuclear positivity was observed in the cells of various tubules/ducts of renal medulla.

Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) - BSA Free [NBP2-31376]

Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis using the Alexa Fluor 488 conjugated FoxO1 antibody, NBP2-31376AF488 (blue) and fluorescence minus one control (red). Staining of FoxO1 in primary CD4+ T-cells isolated from human peripheral blood using 1uL of antibody per 1X10e5 cells. Image from verified customer review.

Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) - BSA Free [NBP2-31376]

Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Using the Alexa Fluor 488 direct conjugate An intracellular stain was performed on Daudi cells with FoxO1/FKHR (83N7F8) antibody NBP2-31376AF488 (blue) and a matched isotype control NBP2-27231AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) - BSA Free [NBP2-31376]

Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - An intracellular stain was performed on U2-OS cells with FoxO1/FKHR [83N7F8] Antibody NBP2-31376AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Simple Western: FoxO1/FKHR Antibody (83N7F8)BSA Free [NBP2-31376]

Simple Western: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Lane view shows a specific band for FoxO1/FKHR in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

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Applications for FoxO1/FKHR Antibody (83N7F8) - BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.1-2 ug/ml

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Simple Western

1:50

Western Blot

0.5-2 ug/ml

Application Notes

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NBP2-31376 in the following applications:

Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FoxO1/FKHR

The FOX1A transcription factor is a member of forkhead family of transcription factors. It is the main target of insulin signaling. When insulin is present, its activity is suppressed. It regulates metabolic homeostasis in response to oxidative stress. It has several other functions: main regulator of redox balance, control osteoblast numbers, promotes gluconeogenesis (hepatocytes), cell death regulation, promote neural cell death, regulation of adipogenic genes, regulate transcriptional activity of GADD45A, and repair nitric oxide-damaged DNA (beta-cells).

Long Name

Forkhead Box O1

Alternate Names

FKH1, FKHR

Entrez Gene IDs

2308 (Human)

Gene Symbol

FOXO1

UniProt

Q12778 (Human)

Additional FoxO1/FKHR Products

Product Documents for FoxO1/FKHR Antibody (83N7F8) - BSA Free

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for FoxO1/FKHR Antibody (83N7F8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

FoxO1/FKHR Antibody (83N7F8) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-31376

Key Product Details

Species Reactivity

Validated:

Cited:

Predicted:

Applications

Validated:

Cited:

Label

Antibody Source

Format

Concentration

View all FoxO1/FKHR Antibodies »

Product Specifications

Immunogen

Reactivity Notes

Localization

Clonality

Host

Isotype

Scientific Data Images for FoxO1/FKHR Antibody (83N7F8) - BSA Free

Western Blot: FoxO1/FKHR Antibody (83N7F8)BSA Free [NBP2-31376]

Immunocytochemistry/ Immunofluorescence: FoxO1/FKHR Antibody (83N7F8) - BSA Free [NBP2-31376]

Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (83N7F8) - BSA Free [NBP2-31376]

Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) - BSA Free [NBP2-31376]

Western Blot: FoxO1/FKHR Antibody (83N7F8)BSA Free [NBP2-31376]

Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (83N7F8) - BSA Free [NBP2-31376]

Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) - BSA Free [NBP2-31376]

Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) - BSA Free [NBP2-31376]

Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) - BSA Free [NBP2-31376]

Simple Western: FoxO1/FKHR Antibody (83N7F8)BSA Free [NBP2-31376]

Applications for FoxO1/FKHR Antibody (83N7F8) - BSA Free

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Immunohistochemistry-Paraffin

Simple Western

Western Blot

Reviewed Applications

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: FoxO1/FKHR

Long Name

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional FoxO1/FKHR Products

Product Documents for FoxO1/FKHR Antibody (83N7F8) - BSA Free

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for FoxO1/FKHR Antibody (83N7F8) - BSA Free