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FOXO3 Antibody (CL13589) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43833

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL13589

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of O43524, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for FOXO3 Antibody (CL13589) - Azide and BSA Free

FOXO3 Antibody (CL13589) - Azide and BSA Free Immunohistochemistry-Paraffin: FOXO3 Antibody [NBP3-43833]

Immunohistochemistry-Paraffin: FOXO3 Antibody [NBP3-43833]

Staining of human anaplastic oligodendroglioma shows strong nuclear positivity in neurons.
FOXO3 Antibody (CL13589) - Azide and BSA Free Immunohistochemistry-Paraffin: FOXO3 Antibody [NBP3-43833]

Immunohistochemistry-Paraffin: FOXO3 Antibody [NBP3-43833]

Staining of human liver shows no nuclear positivity in hepatocytes as expected.
FOXO3 Antibody (CL13589) - Azide and BSA Free Immunohistochemistry-Paraffin: FOXO3 Antibody [NBP3-43833]

Immunohistochemistry-Paraffin: FOXO3 Antibody [NBP3-43833]

Staining of human cerebral cortex shows strong nuclear positivity in neurons.

Applications for FOXO3 Antibody (CL13589) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FoxO3

FOX3 probably functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of the FOXO3 gene with the MLL gene is associated with secondary acute leukemia.

Long Name

Forkhead Box O3

Alternate Names

AF6q21, FKHRL1, FKHRL1P2, FOXO3A

Gene Symbol

FOXO3

Additional FoxO3 Products

Product Documents for FOXO3 Antibody (CL13589) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FOXO3 Antibody (CL13589) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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