Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1A4

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all FOXO4 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 206-505 of human FOXO4 (NP_005929) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

53.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FOXO4 Antibody (OTI1A4) - Azide and BSA Free

Western Blot: FOXO4 Antibody (OTI1A4)Azide and BSA Free [NBP2-71198]

Western Blot: FOXO4 Antibody (OTI1A4)Azide and BSA Free [NBP2-71198]

Western Blot: FOXO4 Antibody (OTI1A4) - Azide and BSA Free [NBP2-71198] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOXO4.
Immunohistochemistry: FOXO4 Antibody (OTI1A4) - Azide and BSA Free [NBP2-71198]

Immunohistochemistry: FOXO4 Antibody (OTI1A4) - Azide and BSA Free [NBP2-71198]

Immunohistochemistry: FOXO4 Antibody (OTI1A4) - Azide and BSA Free [NBP2-71198] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: FOXO4 Antibody (OTI1A4) - Azide and BSA Free [NBP2-71198]

Immunohistochemistry: FOXO4 Antibody (OTI1A4) - Azide and BSA Free [NBP2-71198]

Immunohistochemistry: FOXO4 Antibody (OTI1A4) - Azide and BSA Free [NBP2-71198] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
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Applications for FOXO4 Antibody (OTI1A4) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FOXO4

AFX aka FOXO4 is a forkhead transcription factor involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. FOXO4 down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is also involved in negative regulation of the cell cycle.

Alternate Names

AFX, AFX1myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog);translocated to, 7, Fork head domain transcription factor AFX1, forkhead box O4, forkhead box protein O4, MLLT7MGC120490, myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila);translocated to, 7

Gene Symbol

FOXO4

Additional FOXO4 Products

Product Documents for FOXO4 Antibody (OTI1A4) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for FOXO4 Antibody (OTI1A4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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