Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all FOXO4 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human FOXO4. The immunogen is located within amino acids 30 - 80 of FOXO4. Amino Acid Squence: NQPSEPPEVEPDLGEK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FOXO4 Antibody - BSA Free

Western Blot: FOXO4 AntibodyBSA Free [NBP1-77174]

Western Blot: FOXO4 AntibodyBSA Free [NBP1-77174]

Western Blot: FOXO4 Antibody [NBP1-77174] - HeLa cell lysate with FOXO4 antibody at (A) 0.5 and (B) 1 ug/mL.
FOXO4 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: FOXO4 Antibody - BSA Free [NBP1-77174] -

Immunocytochemistry/ Immunofluorescence: FOXO4 Antibody - BSA Free [NBP1-77174] - Immunocytochemistry of FOXO4 in HeLa cells with FOXO4 antibody at 10 ug/mL.

Applications for FOXO4 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10-20 ug/ml

Western Blot

0.5-1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FOXO4

FOXO4 is a ubiquitously expressed protein member of a subfamily of the forkhead homeotic gene family of transcription factors and shuttles between the cytoplasm and nucleus. FOXO transcription factors are key players of cell fate decisions, metabolism, stress resistance, tumor suppression and are regulated by growth factors, oxidative stress or nutrient deprivation. In the absence of PI3K/AKT activation, FOXO4 localizes in the nucleus where it functions as a transcription factor. FOXO4 can also be phosphorylated by JNK following induction of reactive oxygen species (ROS), resulting in transcriptional activation and the induction of a negative feedback mechanism to counteract the ROS. It is through this mechanism that FOXO4 is thought to sensitize cancer cells to doxorubicin-mediated toxicity.

Alternate Names

AFX, AFX1myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog);translocated to, 7, Fork head domain transcription factor AFX1, forkhead box O4, forkhead box protein O4, MLLT7MGC120490, myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila);translocated to, 7

Gene Symbol

FOXO4

UniProt

NP_005929 (Human)

Additional FOXO4 Products

Product Documents for FOXO4 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FOXO4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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