FoxP3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB600-242

Novus Biologicals, part of Bio-Techne
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NB600-242
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Mouse

Applications

Validated:

Control, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all FoxP3 Antibodies »

Product Specifications

Immunogen

Peptide with sequence SQRPSRCSNPTPGP corresponding to C-Terminus according to NP_054728.2, NP_001107849.1.

Reactivity Notes

Rat reactivity reported from a verified customer review.

Localization

Nuclear (potential).

Specificity

This antibody is expected to recognize both reported isoforms (NP_054728.2 and NP_001107849.1)

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for FoxP3 Antibody

Western Blot: FoxP3 Antibody [NB600-242]

Western Blot: FoxP3 Antibody [NB600-242]

Western Blot: FoxP3 Antibody [NB600-242] - (1 ug/mL) staining of Human Muscle (A), (2 ug/mL) MOLT4 (B) and (1 ug/mL) negative control Pancreas (C) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry: FoxP3 Antibody [NB600-242]

Immunocytochemistry: FoxP3 Antibody [NB600-242]

Immunocytochemistry: FoxP3 Antibody [NB600-242] - (Treg) human blood cells gathered by cytospin and detected by FITC (A) and in phase contrast (B).
Immunohistochemistry: FoxP3 Antibody [NB600-242]

Immunohistochemistry: FoxP3 Antibody [NB600-242]

Immunohistochemistry: FoxP3 Antibody [NB600-242] - (8ug/ml) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.
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Applications for FoxP3 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunohistochemistry-Paraffin

6.0 - 8.0 ug/ml

Peptide ELISA

1:8000

Western Blot

1 - 3 ug/mL
Application Notes
Western blot: Approx 48-50kDa band observed in Human Muscle lysates and in lysates of cell line MOLT4 (calculated MW of 47.2kDa according to NP_054728.2). Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Pancreas lysate
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NB600-242 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FoxP3

FOXP3 (a 381 amino acid protein) is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals. FOXP3 is essential for normal immune homeostasis. FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.

Long Name

Forkhead Box P3

Alternate Names

AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID

Entrez Gene IDs

50943 (Human)

Gene Symbol

FOXP3

UniProt

NP_054728.2 (Human)

Additional FoxP3 Products

Product Documents for FoxP3 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for FoxP3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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