Frataxin Antibody (OTI1C12) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70766

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1C12

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Frataxin Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human FXN(NP_000135) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

18.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Frataxin Antibody (OTI1C12) - Azide and BSA Free

Western Blot: Frataxin Antibody (OTI1C12)Azide and BSA Free [NBP2-70766]

Western Blot: Frataxin Antibody (OTI1C12)Azide and BSA Free [NBP2-70766]

Western Blot: Frataxin Antibody (OTI1C12) - Azide and BSA Free [NBP2-70766] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Frataxin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Frataxin.
Immunocytochemistry/ Immunofluorescence: Frataxin Antibody (OTI1C12) - Azide and BSA Free [NBP2-70766]

Immunocytochemistry/ Immunofluorescence: Frataxin Antibody (OTI1C12) - Azide and BSA Free [NBP2-70766]

Immunocytochemistry/Immunofluorescence: Frataxin Antibody (OTI1C12) - Azide and BSA Free [NBP2-70766] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Frataxin.
Immunohistochemistry: Frataxin Antibody (OTI1C12) - Azide and BSA Free [NBP2-70766]

Immunohistochemistry: Frataxin Antibody (OTI1C12) - Azide and BSA Free [NBP2-70766]

Immunohistochemistry: Frataxin Antibody (OTI1C12) - Azide and BSA Free [NBP2-70766] - Staining of paraffin-embedded Human Kidney tissue using anti-Frataxin mouse monoclonal antibody.
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Applications for Frataxin Antibody (OTI1C12) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Frataxin

Frataxin encodes a mitochondrial protein which belongs to FRATAXIN family. The protein functions in regulating mitochondrial iron transport and respiration. The expansion of intronic trinucleotide repeat GAA results in Friedreich ataxia. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified.

Alternate Names

CyaY, EC 1.16.3.1, FA, FARRmitochondrial, frataxin, Friedreich ataxia, Friedreich ataxia protein, Fxn, X25MGC57199

Gene Symbol

FXN

Additional Frataxin Products

Product Documents for Frataxin Antibody (OTI1C12) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Frataxin Antibody (OTI1C12) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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