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Key Product Details

Species Reactivity

Human, Mouse

Applications

CyTOF-ready, Flow Cytometry, Western Blot

Label

Alexa Fluor 532

Antibody Source

Monoclonal Rat IgG2A Clone # 162531

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Frizzled‑1
Gln72-His248 (Met122Ile)
Accession # O70421

Specificity

Detects mouse Frizzled-1 in direct ELISAs and Western blots. Detects human Frizzled-1 in Flow Cytometry. In direct ELISAs, approximately 30% cross‑reactivity with recombinant mouse (rm) Frizzled-9 is observed and no cross-reactivity with recombinant human (rh) Frizzled-5, rmFrizzled 2, 3, 4, 6, 7, 8, or rhMFRP is observed. No staining is observed on normal human PBMCs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Frizzled-1 Antibody (162531) [Alexa Fluor® 532]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Frizzled-1

FZD1 is a widely expressed Frizzled Receptor that mediates Wnt signaling. FZD1 expression has been reported in heart, kidney, lung, ovary, pancreas, placenta, and prostate. ESTs have been isolated from brain, embryo, eye, heart, heart/melanocyte/uterus, kidney, liver/spleen, lung, prostate, skin, and uterus libraries.

Alternate Names

Frizzled1, FZD1

Gene Symbol

FZD1

Additional Frizzled-1 Products

Product Documents for Frizzled-1 Antibody (162531) [Alexa Fluor® 532]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Frizzled-1 Antibody (162531) [Alexa Fluor® 532]

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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