Frizzled-7 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-93398

Novus Biologicals, part of Bio-Techne
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NB100-93398
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Frizzled-7 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-RFYHRLSHSSKGET corresponding to C-Terminus according to NP_003498.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Frizzled-7 Antibody

Western Blot: Frizzled-7 Antibody [NB100-93398]

Western Blot: Frizzled-7 Antibody [NB100-93398]

Western Blot: Frizzled-7 Antibody [NB100-93398] - Analysis of Frizzled-7 using NB100-93398 at 0.3ug/ml in HepG2 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Frizzled-7 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:64000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 60 kDa band observed in lysates of HEK293 and HepG2 (calculated MW of 63.6 kDa band according to NP_003498.1).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Frizzled-7

Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD7 protein contains an N-terminal signal sequence, 10 cysteine residues typical of the cysteine-rich extracellular domain of Fz family members, 7 putative transmembrane domains, and an intracellular C-terminal tail with a PDZ domain-binding motif. FZD7 gene expression may downregulate APC function and enhance beta-catenin-mediated signals in poorly differentiated human esophageal carcinomas. [provided by RefSeq]

Alternate Names

Frizzled7, FZD7

Entrez Gene IDs

8324 (Human); 14369 (Mouse)

Gene Symbol

FZD7

UniProt

O75084 (Human)

Additional Frizzled-7 Products

Product Documents for Frizzled-7 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Frizzled-7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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