Frizzled-8 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87410

Novus Biologicals, part of Bio-Techne
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NBP1-87410
NBP1-87410-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Frizzled-8 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GSLYSDVSTGLTWRSGTASSVSYPKQMPLSQV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Frizzled-8 Antibody

Western Blot: Frizzled-8 Antibody [NBP1-87410]

Western Blot: Frizzled-8 Antibody [NBP1-87410]

Western Blot: Frizzled-8 Antibody [NBP1-87410] - Analysis in human cell line PC-3 and human cell line HeLa.
Immunohistochemistry-Paraffin: Frizzled-8 Antibody [NBP1-87410]

Immunohistochemistry-Paraffin: Frizzled-8 Antibody [NBP1-87410]

Immunohistochemistry-Paraffin: Frizzled-8 Antibody [NBP1-87410] - Staining of human kidney shows moderate cytoplasmic positivity in tubule cells.

Applications for Frizzled-8 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Frizzled-8

"Frizzled-8 is a receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.

Alternate Names

Frizzled8, FZD8

Gene Symbol

FZD8

Additional Frizzled-8 Products

Product Documents for Frizzled-8 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Frizzled-8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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