FTO Antibody (FT86-4) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80045
![Western Blot: FTO Antibody (FT86-4)Azide and BSA Free [NBP2-80045]](https://resources.bio-techne.com/images/products/FTO-Antibody-FT86-4-Western-Blot-NBP2-80045-img0002.jpg "Western Blot: FTO Antibody (FT86-4)Azide and BSA Free [NBP2-80045]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # FT86-4
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant human FTO.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for FTO Antibody (FT86-4) - Azide and BSA Free
Western Blot: FTO Antibody (FT86-4)Azide and BSA Free [NBP2-80045]
Western Blot: FTO Antibody (FT86-4) [NBP2-80045] - Western blot analysis using anti-FTO (human), mAb (FT86-4) at 1:2'000 dilution.1: Human FTO (His-tagged) (100 ng).2: Mouse FTO (His-tagged) (100 ng).3: Rat FTO (His-tagged) (100 ng).4: HEK 293 cell lysate (100 ug).5: Human Calreticulin (His-tagged) (100 ng) (negative control).
Immunohistochemistry: FTO Antibody (FT86-4) [NBP2-80045]
![Immunoprecipitation: FTO Antibody (FT86-4) - Azide and BSA Free [NBP2-80045]](https://resources.bio-techne.com/images/products/FTO-Antibody-FT86-4-Immunoprecipitation-NBP2-80045-img0001.jpg "Immunoprecipitation: FTO Antibody (FT86-4) - Azide and BSA Free [NBP2-80045]")
Immunoprecipitation: FTO Antibody (FT86-4) - Azide and BSA Free [NBP2-80045]
Immunoprecipitation: FTO Antibody (FT86-4) [NBP2-80045] - Immunoprecipitation of recombinant human FTO using anti-FTO (human), mAb (FT86-4). Recombinant human FTO proteins at different concentrations were precipitated by FT86-4. The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by Western blot with rabbit anti-mouse FTO pAb.Applications for FTO Antibody (FT86-4) - Azide and BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
0.2 um-filtered solution in PBS, pH 7.4
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: FTO
FTO antibodies are useful tools for lipid metabolism research and obesity studies.
Long Name
Fat Mass and Obesity Associated
Alternate Names
Alpha-ketoglutarate-dependent dioxygenase FTO
Gene Symbol
FTO
Additional FTO Products
Product Documents for FTO Antibody (FT86-4) - Azide and BSA Free
Product Specific Notices for FTO Antibody (FT86-4) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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