FUS Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2599

Novus Biologicals, part of Bio-Techne
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NB100-2599
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Bovine (100%), Canine (100%), Monkey (100%), Porcine (100%), Primate (100%), Rabbit (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.25 mg/ml

View all FUS Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human fusion (involved in t(12;16) in malignant liposarcoma) using the numbering given in SwissProt entry P35637 (GeneID 2521).

Reactivity Notes

Panda, Orangutan, Gorilla, Chimpanzee and hirteen-lined ground squirrel (100%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FUS Antibody

Knockout Validated: FUS Antibody [NB100-2599]

Knockout Validated: FUS Antibody [NB100-2599]

Knockout Validated: FUS Antibody [NB100-2599] - Western blot shows lysates of HEK293 human embryonic kidney parental cell line and FUS knockout (KO) HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human FUS Polyclonal Antibody (Catalog # NB100-2599) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for FUS at approximately 75 kDa (as indicated) in the parental HEK293 celll line, but is not detectable in the knockout HEK293 cell line. This experiment was conducted under reducing conditions.
Immunohistochemistry-Paraffin: FUS Antibody [NB100-2599]

Immunohistochemistry-Paraffin: FUS Antibody [NB100-2599]

Immunohistochemistry-Paraffin: FUS Antibody [NB100-2599] - Section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-FUS used at a dilution of 1:100. Detection: DAB
Immunohistochemistry-Paraffin: FUS Antibody [NB100-2599]

Immunohistochemistry-Paraffin: FUS Antibody [NB100-2599]

Immunohistochemistry-Paraffin: FUS Antibody [NB100-2599] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-FUS used at a dilution of 1:500. Detection: DAB staining using anti-rabbit IHC antibody.

Applications for FUS Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Western Blot

1:100-1:2000
Application Notes
Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining. Likely to work with frozen sections. WB reactivity reported in (PMID: 23474818). Use in ICC/IF reported in scientific literature (PMID:33483540)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NB100-2599 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.25 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: FUS

Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Coactivates expression in an agonist- and AF2-dependent manner. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ERs), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Probably functions as a general coactivator, rather than just a nuclear receptor coactivator. May also be involved in the coactivation of the NF-kappa-B pathway. May coactivate expression via a remodeling of chromatin and its interaction with histone acetyltransferase proteins. Involved in placental, cardiac, hepatic and embryonic development. NCOA6 is over-expressed in several breast cancer cell lines.

Alternate Names

75 kDa DNA-pairing protein, ALS6, amyotrophic lateral sclerosis 6, FUS1, fused in sarcoma, fusion (involved in t(12;16) in malignant liposarcoma), fusion gene in myxoid liposarcoma, fusion, derived from t(12;16) malignant liposarcoma, fus-like protein, heterogeneous nuclear ribonucleoprotein P2, HNRNPP2, Oncogene FUS, Oncogene TLS, POMp75, RNA-binding protein FUS, TLShnRNP-P2, Translocated in liposarcoma protein

Entrez Gene IDs

2521 (Human); 233908 (Mouse)

Gene Symbol

FUS

UniProt

P35637 (Human)

Additional FUS Products

Product Documents for FUS Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for FUS Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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