FXR/NR1H4 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-55416
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (100%). Backed by our 100% Guarantee.
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Peptide ELISA
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence KSCREKTELTPDQQ, from the internal region of the protein sequence according to NP_005114.1; NP_001193906.1; NP_001193922.1; NP_001193921.1; NP_001193907.1.
Epitope
KSCREKTELTPDQQ
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for FXR/NR1H4 Antibody
Immunocytochemistry/Immunofluorescence: FXR/NR1H4 Antibody [NB100-55416] -
Immunocytochemistry/Immunofluorescence: FXR/NR1H4 Antibody [NB100-55416] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Flow Cytometry: FXR/NR1H4 Antibody [NB100-55416] -
Flow Cytometry: FXR/NR1H4 Antibody [NB100-55416] - Flow cytometric analysis of paraformaldehyde fixed U2OS cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.Applications for FXR/NR1H4 Antibody
Application
Recommended Usage
Flow Cytometry
10 ug/ml
Immunocytochemistry/ Immunofluorescence
10 ug/ml
Peptide ELISA
Detection limit 1:32000
Application Notes
WB: Preliminary testing showed a band at approx 55kDa in Human Kidney, Colon, Duodenum and Ileum lysate after 0.5ug/ml antibody staining (calculated MW of 54.4kDa according to NP_005114.1). Primary incubation 1 hour at room temperature.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: FXR/NR1H4
Long Name
Farnesoid X-activated receptor
Alternate Names
BAR, HRR-1, NR1H4, RIP14
Entrez Gene IDs
9971 (Human)
Gene Symbol
NR1H4
UniProt
Additional FXR/NR1H4 Products
Product Documents for FXR/NR1H4 Antibody
Product Specific Notices for FXR/NR1H4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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