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FXR/NR1H4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-55416

Novus Biologicals, part of Bio-Techne
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NB100-55416

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence KSCREKTELTPDQQ, from the internal region of the protein sequence according to NP_005114.1; NP_001193906.1; NP_001193922.1; NP_001193921.1; NP_001193907.1.

Epitope

KSCREKTELTPDQQ

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for FXR/NR1H4 Antibody

FXR/NR1H4 Antibody

Immunocytochemistry/Immunofluorescence: FXR/NR1H4 Antibody [NB100-55416] -

Immunocytochemistry/Immunofluorescence: FXR/NR1H4 Antibody [NB100-55416] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
FXR/NR1H4 Antibody

Flow Cytometry: FXR/NR1H4 Antibody [NB100-55416] -

Flow Cytometry: FXR/NR1H4 Antibody [NB100-55416] - Flow cytometric analysis of paraformaldehyde fixed U2OS cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for FXR/NR1H4 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/ml

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Peptide ELISA

Detection limit 1:32000
Application Notes
WB: Preliminary testing showed a band at approx 55kDa in Human Kidney, Colon, Duodenum and Ileum lysate after 0.5ug/ml antibody staining (calculated MW of 54.4kDa according to NP_005114.1). Primary incubation 1 hour at room temperature.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FXR/NR1H4

Bile Acid Receptor NR1H4 is a receptor for bile acids such as chenodeoxycholic acid, lithocholic acid and deoxycholic acid. It represses the transcription of the cholesterol 7 alpha hydroxylase gene and activates the intestinal bile acid binding protein. The NR1H4 gene encodes a protein that has a central role in lipid homeostasis and also regulates carbohydrate metabolism.

Long Name

Farnesoid X-activated receptor

Alternate Names

BAR, HRR-1, NR1H4, RIP14

Entrez Gene IDs

9971 (Human)

Gene Symbol

NR1H4

Additional FXR/NR1H4 Products

Product Documents for FXR/NR1H4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FXR/NR1H4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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