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FXR/NR1H4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16550

Novus Biologicals, part of Bio-Techne
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NBP2-16550

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Predicted:

Bovine (94%), Porcine (92%), Rabbit (96%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human FXR/NR1H4. The exact sequence is proprietary.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FXR/NR1H4 Antibody

Western Blot: FXR/NR1H4 Antibody [NBP2-16550]

Western Blot: FXR/NR1H4 Antibody [NBP2-16550]

Western Blot: FXR/NR1H4 Antibody [NBP2-16550] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NR1H4 antibody.
FXR/NR1H4 Antibody

Immunohistochemistry-Paraffin-NBP2-16550-FXR/NR1H4 Antibody-

Immunohistochemistry-Paraffin-NBP2-16550-FXR/NR1H4 Antibody- Mouse tissues.NR1H4 stained by NR1H4 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
FXR/NR1H4 Antibody

Immunohistochemistry-Paraffin-NBP2-16550-FXR/NR1H4 Antibody-

Immunohistochemistry-Paraffin-NBP2-16550-FXR/NR1H4 Antibody-Rat tissues.NR1H4 stained by NR1H4 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Applications for FXR/NR1H4 Antibody

Application
Recommended Usage

Immunohistochemistry

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Simple Western

Reactivity reported in (PMID: 32189432)

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: FXR/NR1H4

FXR and LXR fall into a category of proteins termed orphan receptors; because of their lack of a defined function, and in the case of LXR, the lack of a defined ligand. FXR has been shown to bind a class of lipid molecules called farnesoids. LXR/RXR heterodimers have highest affinity for DR-4 DNA elements while FXR/RXR heterodimers bind IR-1 elements. Both LXR/RXR and FXR/RXR heterodimers retain their responsiveness to 9-cis retinoic acid. The steroid receptor superfamily acts through direct association with DNA sequences known as hormone response elements (HREs) and bind DNA as either homo- or heterodimers. The promiscuous mediator of heterodimerization, RXR, is the receptor for 9-cis retinoic acid, and dimerizes with VDR, TR, PPAR, as well as several novel receptors including LXR (also referred to as RLD-1) and FXR.

Long Name

Farnesoid X-activated receptor

Alternate Names

BAR, HRR-1, NR1H4, RIP14

Gene Symbol

NR1H4

Additional FXR/NR1H4 Products

Product Documents for FXR/NR1H4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FXR/NR1H4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.