G-CSF Antibody (CSF3/900)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44798
![Immunohistochemistry-Paraffin: G-CSF Antibody (CSF3/900) [NBP2-44798]](https://resources.bio-techne.com/images/products/G-CSF-Antibody-CSF3-900-Immunohistochemistry-Paraffin-NBP2-44798-img0001.jpg "Immunohistochemistry-Paraffin: G-CSF Antibody (CSF3/900) [NBP2-44798]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Rhesus Macaque
Cited:
Human
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Cited:
Immunohistochemistry, Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # CSF3/900
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant full-length human G-CSF protein (Uniprot: P09919)
Reactivity Notes
Macaque Monkey.
Localization
Cytoplasmic
Specificity
This monoclonal antibody recognizes granulocyte-colony stimulating factor (G-CSF) in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell types. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. It reacts with early precursor and mature forms of myeloid cells. It is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes. G-CSF is a pleiotropic cytokine that influences differentiation, proliferation and activation of the neutrophilic granulocyte lineage. The human G-CSF cDNA encodes a 207 amino acid precursor containing a 29 amino acid signal peptide that is proteolytically cleaved to form a 178 amino acid residue mature protein. Two G-CSFs share 73% sequence identity at the amino acid level.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47934)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for G-CSF Antibody (CSF3/900)
Immunohistochemistry-Paraffin: G-CSF Antibody (CSF3/900) [NBP2-44798]
Immunohistochemistry-Paraffin: G-CSF Antibody (CSF3/900) [NBP2-44798] - Human Tonsil stained with G-CSF Monoclonal Antibody (CSF3/900). Note specific cytoplasmic staining of granulocytes.Applications for G-CSF Antibody (CSF3/900)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
1 - 2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: G-CSF
Long Name
Granulocyte Colony Stimulating Factor
Alternate Names
C17orf33, CSF3, CSF3OS, Filgrastim, GCSF, Lenograstim, Pluripoietin
Gene Symbol
CSF3
UniProt
Additional G-CSF Products
Product Documents for G-CSF Antibody (CSF3/900)
Product Specific Notices for G-CSF Antibody (CSF3/900)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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