G protein alpha inhibitor 1 Antibody (2B8-2A5)

Novus Biologicals, part of Bio-Techne | Catalog # H00002770-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2B8-2A5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all G protein alpha inhibitor 1 Antibodies »

Product Specifications

Immunogen

GNAI1 (AAH26326, 1 a.a. ~ 354 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEAGYSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVAAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYQEYAGSNTYEEAAAYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF

Specificity

GNAI1 - guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for G protein alpha inhibitor 1 Antibody (2B8-2A5)

Western Blot: G protein alpha inhibitor 1 Antibody (2B8-2A5) [H00002770-M01]

Western Blot: G protein alpha inhibitor 1 Antibody (2B8-2A5) [H00002770-M01]

Western Blot: G protein alpha inhibitor 1 Antibody (2B8-2A5) [H00002770-M01] - Analysis of GNAI1 expression in transfected 293T cell line by GNAI1 monoclonal antibody (M01), clone 2B8-2A5.Lane 1: GNAI1 transfected lysate(40.4 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: G protein alpha inhibitor 1 Antibody (2B8-2A5) [H00002770-M01]

Immunohistochemistry-Paraffin: G protein alpha inhibitor 1 Antibody (2B8-2A5) [H00002770-M01]

Immunohistochemistry-Paraffin: G protein alpha inhibitor 1 Antibody (2B8-2A5) [H00002770-M01] - Analysis of monoclonal antibody to GNAI1 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.
Western Blot: G protein alpha inhibitor 1 Antibody (2B8-2A5) [H00002770-M01]

Western Blot: G protein alpha inhibitor 1 Antibody (2B8-2A5) [H00002770-M01]

Western Blot: G protein alpha inhibitor 1 Antibody (2B8-2A5) [H00002770-M01] - GNAI1 monoclonal antibody (M01), clone 2B8-2A5. Analysis of GNAI1 expression in human liver.
View 1 more images

Applications for G protein alpha inhibitor 1 Antibody (2B8-2A5)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: G protein alpha inhibitor 1

Guanine nucleotide-binding proteins (G proteins) form a large family of signal-transducing molecules. They are found as heterotrimers made up of alpha, beta, and gamma subunits. Members of the G protein family have been characterized most extensively on the basis of the alpha subunit, which binds guanine nucleotide, is capable of hydrolyzing GTP, and interacts with specific receptor and effector molecules. The G protein family includes Gs (MIM 139320) and Gi, the stimulatory and inhibitory GTP-binding regulators of adenylate cyclase; Go, a protein abundant in brain (GNAO1; MIM 139311); and transducin-1 (GNAT1; MIM 139330) and transducin-2 (GNAT2; MIM 139340), proteins involved in phototransduction in retinal rods and cones, respectively (Sullivan et al., 1986 [PubMed 3092218]; Bray et al., 1987 [PubMed 3110783]). Suki et al. (1987) [PubMed 2440724] concluded that the human genome contains at least 3 nonallelic genes for alpha-i-type subunits of G protein; see, e.g, GNAI2 (MIM 139360), GNAI3 (MIM 139370), and GNAIH (MIM 139180).[supplied by OMIM]

Alternate Names

Adenylate cyclase-inhibiting G alpha protein, Gi, Gi1 protein alpha subunit, guanine nucleotide binding protein (G protein), alpha inhibiting activitypolypeptide 1, guanine nucleotide-binding protein G(i) subunit alpha-1

Entrez Gene IDs

2770 (Human)

Gene Symbol

GNAI1

OMIM

139310 (Human)

UniProt

AAH26326 (Human)

Additional G protein alpha inhibitor 1 Products

Product Documents for G protein alpha inhibitor 1 Antibody (2B8-2A5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for G protein alpha inhibitor 1 Antibody (2B8-2A5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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