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G3BP1 Antibody (HL2254) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25472

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25472

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2254

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for G3BP1 Antibody (HL2254) - Azide and BSA Free

Immunogen

Recombinant fragment of human G3BP1.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for G3BP1 Antibody (HL2254) - Azide and BSA Free

G3BP1 Antibody (HL2254) - Azide and BSA Free

Immunohistochemistry-Paraffin: G3BP1 Antibody (HL2254) - Azide and BSA Free [NBP3-25472] -

Immunohistochemistry-Paraffin: G3BP1 Antibody (HL2254) - Azide and BSA Free [NBP3-25472] - G3BP1 antibody [HL2254] detects G3BP1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. G3BP1 stained by G3BP1 antibody [HL2254] (NBP3-25472) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
G3BP1 Antibody (HL2254) - Azide and BSA Free

Western Blot: G3BP1 Antibody (HL2254) - Azide and BSA Free [NBP3-25472] -

Western Blot: G3BP1 Antibody (HL2254) - Azide and BSA Free [NBP3-25472] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with G3BP1 antibody [HL2254] (NBP3-25472) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
G3BP1 Antibody (HL2254) - Azide and BSA Free

Western Blot: G3BP1 Antibody (HL2254) - Azide and BSA Free [NBP3-25472] -

Western Blot: G3BP1 Antibody (HL2254) - Azide and BSA Free [NBP3-25472] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with G3BP1 antibody [HL2254] (NBP3-25472) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for G3BP1 Antibody (HL2254) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: G3BP1

G3BP1 encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Alternate Names

ATP-dependent DNA helicase VIII, EC 3.6.1, EC 3.6.4.12, EC 3.6.4.13, G3BP-1, G3BPRas-GTPase-activating protein SH3-domain-binding protein, GAP binding protein, GAP SH3 domain-binding protein 1, GTPase activating protein (SH3 domain) binding protein 1, hDH VIII, HDH-VIII, MGC111040, ras GTPase-activating protein-binding protein 1, RasGAP-associated endoribonuclease G3BP

Gene Symbol

G3BP1

Additional G3BP1 Products

Product Documents for G3BP1 Antibody (HL2254) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for G3BP1 Antibody (HL2254) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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